Transformation-enhancing compositions and methods of use
AssigneePioneer Hi-Bred International, Inc.
InventorsMargit C. Ross, Laura A. Church, William J. Gordon-Kamm
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Abstract
The invention relates to compositions and methods and for genetically transforming Zea mays plants. Compositions comprising transformation-enhancing agents and methods of use are provided. The methods involve administering an effective amount of a transformation-enhancing agent to a Zea mays plant. The compositions and methods of the invention find use in improving transformation efficiency and increasing the embryogenic response of callus.
Claims24
A method for transforming a Zea mays plant, said method comprising:
- (a) administering, in planta, an effective amount of a transformation-enhancing agent by injecting into said Zea mays plant or applying topically to said Zea mays plant, said transformation-enhancing agent comprising at least one component selected from the group consisting of an auxin, an auxin analog, a cytokinin, an ABA synthesis inhibitor, an antioxidant, an inhibitor of ethylene synthesis, an inhibitor of ethylene action, Medium 604, a hormone cocktail, proline mix, salts cocktail 1 and ABA;
- (b) transforming a cell from said Zea mays plant with a DNA construct comprising a nucleotide sequence of interest;
- and
- (c) regenerating a transformed Zea mays plant from said cell.
A method for increasing Zea mays plant transformation efficiency above the level of a control, said method comprising:
- (a) administering, in planta, an effective amount of a transformation-enhancing agent by injecting into said Zea mays plant or applying topically to said Zea mays plant, said transformation-enhancing agent comprising at least one component selected from the group consisting of an auxin, an auxin analog, a cytokinin, an ABA synthesis inhibitor, an antioxidant, an inhibitor of ethylene synthesis, an inhibitor of ethylene action, Medium 604. a hormone cocktail, proline mix, salts cocktail 1 and ABA;
- (b) transforming a cell of said Zea mays plant with a DNA construct comprising a nucleotide sequence of interest;
- and
- (c) regenerating a transformed Zea mays plant from said cell.
A method for transforming a Zea mays plant embryo, said method comprising:
- (a) administering, in planta, an effective amount of a transformation-enhancing agent by injecting into said Zea mays plant or applying topically to said Zea mays plant, said transformation-enhancing agent comprising at least one component selected from the group consisting of an auxin, an auxin analog, a cytokinin, an ABA synthesis inhibitor, an antioxidant, an inhibitor of ethylene synthesis, an inhibitor of ethylene action, Medium 604, a hormone cocktail, proline mix, salts cocktail 1 and ABA;
- (b) transforming a cell from an embryo produced by said Zea mays plant with a DNA construct comprising a nucleotide sequence of interest;
- and
- (c) regenerating said cell into a transformed Zea mays embryo or a transformed Zea mays shoot.
A method for transforming a Zea mays plant, said method comprising:
- (a) administering, in planta, an effective amount of a transformation-enhancing agent by injecting into said plant said transformation-enhancing agent consisting of water;
- (b) transforming a cell from said plant with a DNA construct comprising a nucleotide sequence of interest;
- and
- (c) regenerating a transformed plant from said cell.
Dependencies are inferred from the claim text.