Microfluidic devices and methods for separation
AssigneeCaliper Technologies Corp.
InventorsMichael Knapp, John Wallace Parce, Luc J. Bousse, Anne R. Kopf-Sill
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Abstract
Integrated systems, apparatus, software, and methods for performing biochemical analysis, including DNA sequencing, genomic screening, purification of nucleic acids and other biological components and drug screening are provided. Microfluidic devices, systems and methods for using these devices and systems for performing a wide variety of fluid operations are provided. The devices and systems of are used in performing fluid operations which require a large number of iterative, successive or parallel fluid manipulations, in a microscale, or sealed and readily automated format.
Claims22
A non-electrophoretic method of separating material in a microfluidic device, the method comprising:
- flowing at least a first and a second material into contact in the microfluidic device;
- flowing the first material, the second material, or a reaction product resulting from contact of the first and second materials into a microchannel comprising at least one separation region comprising a chromatographic material, wherein the chromatographic material comprises a size-separation matrix, an affinity matrix, a gel-exclusion matrix, or a combination thereof, and whereby the first material, the second material, or the reaction product is separated from at least one other material in the separation region;
- and
- detecting a resulting separated product.
A microfluidic device for non-electrophoretic separation of materials, comprising:
- a body structure comprising at least first and second intersecting microchannels;
- a chromatographic material deposited in or fluidically coupled to at least a first portion of at least one of the two microchannels, wherein the chromatographic material comprises a size-separation matrix, an affinity matrix, a gel-exclusion matrix, or a combination thereof;
- and
- a material transport system fluidically coupled to the first portion, wherein, during operation of the device, the material transport system forces fluid through the first portion.
Dependencies are inferred from the claim text.