Methods for identifying herbicidal agents that inhibit D1 protease
AssigneeE. I. Du Pont de Nemours and Company
InventorsDexter Allan Chisholm, Bruce Aaron Diner, Gail K Donaldson, Howard Paul Hershey, Douglas Brian Jordan, Xiao Song Tang, Shaojie Wang
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Abstract
D1 protease has been isolated from the alga (Scenedesmjus obliquus), wheat, and Synechocystis PCC 6803 and the genes encoding these enzymes have been cloned and sequenced. Native or recombinantly produced enzyme has been used to develop assays to detect herbicidal compositions capable of inhibiting the D1 protease enzyme.
Claims6
An isolated nucleic acid fragment encoding a D1 protease enzyme, the enzyme having the amino acid sequence selected from the group consisting of SEQ ID NO:9, SEQ ID NO:15, SEQ ID NO:4, and SEQ ID NO:13.
An isolated nucleic acid fragment encoding a D1 protease enzyme selected from the group of nucleotide sequences consisting of SEQ ID NO:8, SEQ ID NO:14, SEQ ID NO:3, and SEQ ID NO:12.
A transformed E. coli cell corresponding to the biological deposit designated ATCC 98186 comprising an isolated nucleic acid fragment encoding the wheat D1 protease enzyme.
A method for the recombinant production of enzymatically active D1 protease enzyme comprising:
- (a) transforming a suitable host cell with a vector comprising an isolated nucleic acid fragment encoding a D1 protease enzyme, the enzyme having an amino acid sequence selected from the group consisting of SEQ ID NO:9, SEQ ID NO:15, SEQ ID NO:4, and SEQ ID NO:13, the isolated nucleic acid fragment operably linked to at least one suitable regulatory sequence;
- (b) culturing the transformed cell of step (a) under conditions wherein D1 protease is expressed;
- and
- (c) recovering the D1 protease expressed in step (b).
Dependencies are inferred from the claim text.