Method for increasing the gene expression of saccharose synthase
AssigneeForschungszentrum Julich GmbH
InventorsWolf-Bernd Frommer, Henning Schrader, Lothar Elling
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Abstract
The invention relates to a method for increasing the gene expression of saccharose synthase. To this end the invention provides for the expression of the saccharose synthase gene to be placed under the control of a proton-ATPase promoter. According to a preferred version of the invention gene expression of the saccharose synthase is also increased by increasing the number of copies of the saccharose synthase gene and of the proton-ATPase promoter.
Claims15
A process for increasing gene expression of saccharose synthase in which the expression of the saccharose synthase gene is carried out in a transformed yeast cell containing a saccharose synthase gene wherein the saccharose synthase gene is under the control of a proton-ATPase promotor derived from yeast and capable of producing saccharose synthase having an activity of about 6.2 U/g of yeast cells or of about 0.22 U/mg of total protein in the raw decomposed yeast cells and in which the saccharose synthase is free from invertase activity.
The saccharose synthase gene preceded by a proton ATPase promotor obtained from a yeast and capable of producing saccharose synthase having an activity of about 6.2 U/g of yeast cells or of about 0.22 U/mg of total protein in the raw decomposed yeast cells.
A process for increasing gene expression of saccharose synthase in which the expression of the saccharose synthase gene is carried out in a transformed Saccharomyces cerevisiae yeast cell containing a saccharose synthase gene derived from Solanum tuberosum wherein the saccharose synthase gene is under the control of a proton-ATPase promotor derived from Saccharomyces cerevisiae and in which the saccharose synthase is free from invertase activity.
Dependencies are inferred from the claim text.