D-sorbitol dehydrogenase gene
AssigneeRoche Vitamins Inc.
InventorsTatsuo Hoshino, Taro Miyazaki, Setsuko Ojima, Masako Shinjoh, Noribumi Tomiyama
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Abstract
Polynucleotides encoding proteins with D-sorbitol dehydrogenase activity or which function to activate D-sorbitol dehydrogenase proteins, in vivo, vectors and recombinant organisms which contain such polynucleotides, and methods of making D-sorbitol dehydrogenase and L-sorbose.
Claims39
A process for producing L-sorbose which comprises converting D-sorbitol into L-sorbose by fermentation, in an appropriate medium, of a microorganism containing a recombinant polynucleotide fragment comprising a polynucleotide sequence that encodes a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 6, 6, 8, 9, and combinations thereof, which fragment encodes a D-sorbitol dehydrogenase obtained from Gluconobacter suboxydans.
A process for producing L-sorbose comprising cultivating a microorganism comprising a recombinant polynucleotide sequence encoding the amino acid residues of SEQ ID NO:
- 2.
A process for producing L-sorbose comprising cultivating a microorganism in a medium having D-sorbitol, the microorganism comprising a polynucleotide sequence having the nucleotides identified in SEQ ID NO:
- 1 or a fragment of SEQ ID NO:
- 1 encoding D-sorbitol dehydrogenase activity.
A process for producing L-sorbose comprising cultivating a microorganism in a medium having D-sorbitol, the microorganism comprising a recombinant polynucleotide sequence encoding D-sorbitol dehydrogenase activity, which polynucleotide sequence consists of nucleotides 192 to 569 and nucleotides 644 to 2791 of SEQ ID NO:
- 1.
A process for producing L-sorbose comprising cultivating a microorganism in a medium having D-sorbitol, the microorganism comprising a recombinant polynucleotide sequence encoding D-sorbitol dehydrogenase activity, which polynucleotide sequence consists of nucleotides 192 to 569 and nucleotides 572 to 2791 of SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.