Calcium binding proteolipid compositions and methods
AssigneeBoard of Regents, The University of Texas System
InventorsBarbara D. Boyan, Simon Van Dijk, David D. Dean
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Abstract
The invention relates to the cloning and sequencing of the DNA encoding a membrane associated proteolipid from C. matruchotii that is capable of inducing in vitro calcium binding. A proteolipid was extracted from cultures of C. matruchotii and separated into a lipid component and three apoproteins having molecular weights of approximately 5.0, 5.5 and 7.5 kDa as determined by SDS-PAGE. The invention also includes the polyclonal and monoclonal antibodies directed against the membrane associated proteolipid and immunological assays developed with these antibodies.
Claims10
A method of preparing a polypeptide, comprising:
- (a) growing a recombinant host cell under conditions permitting nucleic acid expression and polypeptide production;
- wherein said recombinant host cell comprises a vector comprising an isolated nucleic acid sequence that encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:1 or SEQ ID NO:8;
- and
- (b) recovering the polypeptide so produced.
Dependencies are inferred from the claim text.