Detection of 2-methylisoborneol by monoclonal antibody production

Abstract

We have produced a hybridoma cell line that produces monoclonal antibodies (MAb) that bind effectively to 2-methylisoborneol (MIB). The MAbs were produced using a structurally related molecule, borneol, whose structure was minimally changed when complexed to the protein carrier. These MAbs detected MIB at approximately 0.01 to 1 ppb (or lower) when used in ELISA assays. Because these antibodies were produced as MAbs, their affinity characteristics remained constant. With access to MAb's, MIB can be easily detected at low levels using immunological techniques that are adapted to fast and easy field assays of large samples. For example, catfish could easily be assayed in the field for the presence of MIB prior to marketing.

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