Production and use of normalized DNA libraries
AssigneeDiversa Corporation
InventorsJay M. Short, Eric J. Mathur
Life of this patent
Estimating the term…
Abstract
Disclosed is a process for forming a normalized genomic DNA library from an environmental sample by (a) isolating a genomic DNA population from the environmental sample; (b) at least one of (i) amplifying the copy number of the DNA population so isolated and (ii) recovering a fraction of the isolated genomic DNA having a desired characteristic; and (c) normalizing the representation of various DNAs within the genomic DNA population so as to form a normalized library of genomic DNA from the environmental sample. Also disclosed is a normalized genomic DNA library formed from an environmental sample by the process.
Claims19
A method for forming a normalized DNA library from a mixed population of organisms, which comprises:
- (a) obtaining a DNA population from the mixed population of organisms;
- (b) at least one of the steps selected from the group consisting of (i) amplifying the copy number of the DNA population so isolated and (ii) recovering a fraction of the isolated DNA having a desired characteristic;
- and
- (c) normalizing the representation of various DNAs within the DNA population so as to form a normalized library of DNA from the mixed population of organisms.
A normalized DNA library formed from a mixed population of organisms by a method comprising:
- (a) obtaining a DNA population from the mixed population of organisms;
- (b) at least one of (i) amplifying the copy number of the DNA population so isolated and (ii) recovering a fraction of the isolated DNA having a desired characteristic;
- (c) normalizing the representation of various DNAs within the DNA population;
- and
- (d) transforming host cells with the DNA of (c) so as to form a normalized library of DNA from the mixed population of organisms.
A method for producing a normalized library of gene clusters from a mixed population of organisms, which comprises:
- (a) obtaining a DNA population from the mixed population of organisms;
- (b) at least one of (i) amplifying the copy number of the DNA population so isolated and (ii) recovering a fraction of the isolated DNA having a desired characteristic;
- and
- (c) normalizing the representation of various DNAs within the DNA population so as to produce a normalized library of DNA from the mixed population of organisms.
A normalized library of gene clusters formed from a mixed population of organisms by a method comprising:
- (a) obtaining a DNA population from the mixed population of organisms;
- (b) at least one of (i) amplifying the copy number of the DNA population so isolated and (ii) recovering a fraction of the isolated DNA having a desired characteristic;
- and
- (c) normalizing the representation of various DNAs within the DNA population so as to form a normalized library of DNA from the mixed population of organisms.
Dependencies are inferred from the claim text.