Microstamping activated polymer surfaces
AssigneeDuke University
InventorsAshutosh Chilkoti, Zhongping Yang
Life of this patent
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Abstract
Functionalized polymer surfaces having reactive moieties thereon are contacted with stamps having ligands adsorbed thereto, the ligands also comprising reactive moieties. The reactive moieties of the functionalized surfaces and the ligands form covalent bonds, thus providing a method of microstamping polymer surfaces directly with ligands such as biological ligands. Using this method, devices such as tissue culture plates with polymer surfaces that are microstamped directly with ligands can be made.
Claims32
A method of microstamping a polymer surface with a biological ligand, comprising:
- introducing a reactive moiety onto the polymer surface to provide a functionalized polymer surface, wherein said step of introducing a reactive moiety onto the polymer surface comprises at least one method selected from the group consisting of hydrolysis, reduction, photoinitiated graft polymerization, aminolysis, forming a surface interpenetrating network of poly(ethylene oxide), chemical reaction at hydroxyl end-groups, corona discharge, reactive plasma etching, laser treatment, and ion beam modification;
- contacting the functionalized polymer surface having the first reactive moiety thereon with a stamp that has adsorbed onto its surface at least one biological ligand comprising a second reactive moiety, wherein the second reactive moiety of the biological ligand and the first reactive moiety of the functionalized polymer surface form a covalent bond;
- and then
- separating the stamp from the functionalized polymer surface, thereby leaving the biological ligand directly and covalently bound to the functionalized polymer surface.
method of forming a device comprising at least one microstamped polymer surface, wherein the polymer surface is covalently bound to at least one ligand, comprising:
- introducing a reactive moiety onto the polymer surface of a device to provide a functionalized polymer surface, wherein said step of introducing a reactive moiety onto the polymer surface comprises at least one method selected from the group consisting of hydrolysis, reduction, photoinitiated graft polymerization, aminolysis, forming a surface interpenetrating network of poly(ethylene oxide), chemical reaction at hydroxyl end-groups, corona discharge, reactive plasma etching, laser treatment, and ion beam modification;
- contacting the functionalized polymer surface with a stamp that has adsorbed onto its surface at least one biological ligand comprising a second reactive moiety that binds the first reactive moiety on the functionalized polymer surface, wherein the second reactive moiety of the biological ligand and the first reactive moiety of the functionalized polymer surface of the device form a covalent bond;
- and then
- separating the stamp from the at least one polymer whose surface is functionalized of the device, thereby leaving the biological ligand covalently and directly bound to the first reactive moiety on the polymer surface of the device.
Dependencies are inferred from the claim text.