Canola oil having increased oleic acid and decreased linolenic acid content
AssigneeCargill, Incorporated
InventorsLorin R. DeBonte, William D. Hitz
Life of this patent
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Abstract
An endogenous oil extracted from Brassica seeds is disclosed that contains, after crushing and extraction, greater than 86% oleic acid and less than 2.5% α-linolenic acid. The oil also contains less than 7% linoleic acid. The Brassica seeds are produced by plants that contain seed-specific inhibition of microsomal oleate desaturase and microsomal linoleate desaturase gene expression. Such inhibition can be created by cosuppression or antisense technology. Such an oil has a very high oxidative stability in the absence of added antioxidants.
Claims24
A Brassica plant containing at least one recombinant nucleic construct, said at least one construct comprising:
- a) a first seed-specific regulatory sequence fragment operably linked to a wild-type microsomal delta-12 fatty acid desaturase coding sequence fragment;
- and
- b) a second seed-specific regulatory sequence fragment operably linked to a wild-type microsomal delta-15 fatty acid desaturase coding sequence fragment,
- wherein said plant produces seeds, yielding an oil having an oleic acid content of about 86% or greater and an erucic acid content of less than about 2%, said oleic acid content and erucic acid content determined after hydrolysis of said oil.
A Brassica plant containing at least one recombinant nucleic acid construct, said at least one construct comprising:
- a) a first seed-specific regulatory sequence fragment operably linked to a wild-type microsomal delta-12 fatty acid desaturase coding sequence fragment;
- and
- b) a second seed-specific regulatory sequence fragment operably linked to a wild-type microsomal delta-15 fatty acid desaturase coding sequence fragment, wherein said plant produces seeds yielding an oil having an oleic acid content of 80% or greater, an α-linolenic acid content of about 2.5% or less, and an erucic acid content of less than about 2%, said oleic acid content, linolenic acid content and erucic acid content determined after hydrolysis of said oil.
A method of producing an oil from Brassica seeds, said method comprising:
- a) crushing seeds of at least one transgenic Brassica plant, said Brassica plant containing at least one recombinant nucleic acid construct, said at least one construct comprising a first seed-specific regulatory sequence fragment operably linked to a wild-type delta-12 fatty acid desaturase coding sequence fragment and a second seed-specific regulatory sequence fragment operably linked to a wild-type delta-15 fatty acid desaturase coding sequence fragment;
- and
- b) extracting said oil from said crushed seeds, said oil having an oleic acid content of about 80% or greater, an α-linolenic acid content of 2.5% or less, and an erucic acid content of less than 2%, said oleic acid, linolenic acid, and erucic acid content determined after hydrolysis of said oil.
Dependencies are inferred from the claim text.