Diaminofluorescein derivative
AssigneesDaiichi Pure Chemicals Co., Ltd., Tetsuo Nagano
InventorsTetsuo Nagano, Hirotatsu Kojima
Life of this patent
Estimating the term…
Abstract
A compound represented by the following formula (I): wherein R1 and R2 represent amino group that substitute at adjacent positions on the phenyl ring, provided that either of R1 and R2 represents a mono(C1-6 alkyl)-substituted amino group and the other represents an unsubstituted amino group; and R3 and R4 independently represent hydrogen atom or an acyl group, and an agent for measurement of nitrogen monoxide which comprises said compound.
Claims11
A compound represented by the following formula (I):
- wherein R1 and R2 represent amino groups that substitute at adjacent positions on the phenyl ring, provided that either of R1 and R2 represents a mono(C1-6 alkyl)-substituted amino group and the other represents an unsubstituted amino group;
- and R3 and R4 independently represent hydrogen atom or an acyl group.
A compound represented by the following formula (II):
- wherein R11 and R12 combine together to form a group represented by —N═N—N(R19)— which forms a ring structure at adjacent positions on the phenyl ring, wherein R19 represents a C1-6 alkyl group, or R11 and R12 represent a combination of an amino group and a nitro group which substitute at adjacent positions on the phenyl ring;
- and R13 and R14 independently represent hydrogen atom or an acyl group.
A method for measuring nitrogen monoxide which comprises:
- reacting a compound represented by formula (I) with nitrogen monoxide to form a compound represented by formula (II);
- irradiating the compound represented by formula (II) with light to cause fluorescence of the irradiated compound;
- and
- measuring the fluorescence;
- wherein the compound represented by formula (I) has the following formula:
- wherein R1 and R2 represent amino groups that substitute at adjacent positions on the phenyl ring, provided that either of R1 and R2 represents a mono(C1-6 alkyl)-substituted amino group and the other represents an unsubstituted amino group and wherein R3 and R4 independently represent hydrogen atom or an acyl group;
- and
- wherein the compound represented by formula (II) has the following formula:
- wherein R11 and R12 combine together to form a group represented by —N═N—N(R19)— which forms a ring structure at adjacent positions on the phenyl ring, wherein R19 represents a C1-6 alkyl group, or R11 and R12 represent a combination of an amino group and a nitro group which substitute at adjacent positions on the phenyl ring;
- and R13 and R14 independently represent hydrogen atom or an acyl group.
Dependencies are inferred from the claim text.