Separating plasmids from contaminants using hydrophobic or hydrophobic and ion exchange chromatography

A method for separating a plasmid from a solution containing the plasmid, together with contaminants selected from a group consisting of proteins, RNAs, DNAs and mixtures thereof, using hydrophobic interaction chromatography, comprising the steps of

• a) selecting a first column material for a first hydrophobic interaction chromatography that does not adsorb the plasmid at a salt concentration at which the protein and RNA are adsorbed,
• b) selecting a second column material for a second hydrophobic interaction chromatography that adsorbs the plasmid at a salt concentration at which the first column material adsorbs protein and RNA and the first column material does not adsorb the plasmid,
• c) preparing a tandem column by connecting in series a first column containing the first column material to a second column containing the second column material,
• d) injecting the solution containing the plasmid and contaminants into the tandem column to adsorb protein and RNA onto the first column material and plasmid and DNA onto the second column material,
• e) breaking the connection between the first column and the second column, and
• f) eluting the adsorbed plasmid from the second column material with an eluent containing salt of a concentration at which plasmid is eluted from the second column material.

A method for separating a plasmid from a solution containing the plasmid, together with contaminants selected from a group consisting of proteins, RNAs, DNAs and mixtures thereof, using hydrophobic interaction chromatography and ion exchange chromatography, comprising the steps of

• a) selecting a first column material for a first hydrophobic interaction chromatography that does not adsorb the plasmid at a salt concentration at which protein and RNA are adsorbed,
• b) selecting a second column material for a second hydrophobic interaction chromatography that adsorbs the plasmid at a salt concentration at which the first column material adsorbs protein and RNA and the first column material does not adsorb the plasmid,
• c) injecting the solution containing the plasmid and contaminants into a first column containing the first column material to adsorb the protein and RNA onto the first column material and form an eluate containing the plasmid and DNA,
• d) injecting the eluate, containing the plasmid and DNA, from the first column into a second column containing the second column material to adsorb the plasmid and DNA onto the second column material,
• e) eluting the adsorbed plasmid from the second column material with an eluent containing salt of a concentration at which the plasmid is eluted from the second column material in an eluate,
• f) injecting the eluate into a column for ion exchange chromatography containing a material for ion exchange chromatography at a salt concentration at which the material for ion exchange chromatography adsorbs the plasmid, to adsorb the plasmid onto the material for ion exchange chromatography, and
• g) eluting the adsorbed plasmid from the material for ion exchange chromatography with an eluent containing salt of a concentration at which the plasmid is eluted from the material for ion exchange chromatography.

A method for separating a plasmid from a solution containing the plasmid, together with contaminants selected from a group consisting of protein, RNA, DNA and mixtures thereof, using hydrophobic interaction chromatography and ion exchange chromatography, comprising the steps of

• a) selecting a first column material for a first hydrophobic interaction chromatography that does not adsorb the plasmid at a salt concentration at which protein and RNA are adsorbed,
• b) selecting a second column material for a second hydrophobic interaction chromatography that adsorbs the plasmid at a salt concentration at which the first column material adsorbs protein and RNA and the first column material does not adsorb the plasmid,
• c) preparing a tandem column by connecting in series a first column containing the first column material to a second column containing the second column material,
• d) injecting the solution containing the plasmid and contaminants into the tandem column to adsorb the protein and RNA onto the first column material and plasmic and DNA onto the second column material,
• e) beaking the connection between the first column and the second column,
• f) eluting the adsorbed plasmid from the second column material with an eluent containing salt of a concentration at which the plasmid is eluted from the second column material in an eluate,
• g) injecting the eluate containing the plasmid from the second column into a column for the ion exchange chromatography containing a meterial for ion exchange chromatography, at a salt concentration at which the material for ion exchange chromatography adsorbs plasmid, to adsorb the plasmid onto the material for the ion exchange chromatography, and
• h) eluting the adsorbed plasmid from the material for ion exchange chromatography with an eluent containing salt of a concentration at which the plasmid is eluted from the material for ion exchange chromatography.