Nucleic acid sequences encoding protein allergens of the species Cynodon dactylon
AssigneeThe University of Melbourne
InventorsMohan Bir Singh, Penelope Smith, Robert Bruce Knox
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Abstract
The present invention provides nucleic acid sequences coding Cyn dI, or at least one fragment thereof or the functional equivalent of such nucleic acid sequences. The present invention also provides expression vectors comprising such nucleic acid sequences and host cells transformed therewith. The present invention further provides isolated Bermuda grass pollen protein allergen Cyn dI or fragments thereof. Isolated Bermuda grass pollen protein allergens or antigenic or allergenic fragments thereof are useful for diagnosing and treating sensitivity in an individual to Bermuda grass pollen allergens.
Claims31
An isolated nucleic acid sequence coding for a Bermuda grass pollen protein allergen, Cyn dI, wherein the Cyn dI protein allergen comprises an amino acid sequence selected from the group consisting of Cyn dI.18 (SEQ ID No:
- 15), Cyn dI.CD1 (SEQ ID NO:
- 21) and Cyn dI2/3 (SEQ ID NO:
- 24), all as shown in FIG. 20.
An isolated nucleic acid sequence coding for a Bermuda grass pollen protein allergen Cyn dI, having the formula:
- L1NYX
- wherein L1 is a nucleic acid sequence of 0-300 nucleotides which nucleic acid sequence includes nucleotides encoding a leader sequence of Cyn dI, N is a nucleic acid sequence comprising up to 600 nucleotides which nucleic acid sequence contains nucleotides encoding the amino terminus portion of mature Cyn dI, Y is the portion of the nucleic acid sequence of clone 2 (SEQ ID NO:
- 1), clone 18 (SEQ ID NO:
- 3), clone 3 (SEQ ID NO:
- 17), clone 22 (SEQ ID NO:
- 18), clone 23 (SEQ ID NO:
- 19) or any polymorphic form thereof that codes for mature Cyn dI, and X is a nucleic acid sequence of 0-600 nucleotides which nucleic acid sequence contains nucleotides of the 3′ untranslated portion of Cyn dI, and wherein L1 and X can be 0.
An isolated nucleic acid sequence coding for a Bermuda grass pollen protein allergen, Cyn dI, wherein the Cyn dI allergen is encoded by the nucleic acid sequence of clone CD1 as shown in FIG. 18 (SEQ ID NO:
- 20), or a portion thereof encoding at least one T cell epitope of Cyn dI.
An isolated nucleic acid sequence coding for a Bermuda grass pollen allergen, Cyn dI, wherein the Cyn dI allergen comprises the amino acid sequence of Cyn dI.2/3 (full-length) as shown in FIG. 20 (SEQ ID NO:
- 24), or a portion thereof encoding at least one T cell epitope of Cyn dI.
Dependencies are inferred from the claim text.