Calcium channel compositions and methods of use thereof
AssigneeThe United States of America as represented by the Department of Health and Human Services
InventorsMichael Isaac Lerman, Farida Latif, Ming-Hui Wei, Fuh-Mei Duh, John Dorrance Minna, Yoshitaka Sekido, Boning Gao
Life of this patent
Estimating the term…
Abstract
The present invention relates to calcium channel compositions and methods of making and using same. In particular, the invention relates to calcium channel alpha2delta (α2δ) subunits and nucleic acid sequences encoding them. These compositions are useful in methods for identifying compounds that modulate the activity of calcium channels and for identifying compounds as therapeutic for disease.
Claims18
A purified nucleic acid sequence comprising a nucleotide sequence selected from the group consisting of (a) SEQ ID NO:1 and the complement thereof, (b) SEQ ID NO:3 and the complement thereof, and (c) SEQ ID NO:5 and the complement thereof.
A method for producing a calcium channel protein, comprising:
- a) providing:
- i) a recombinant expression vector comprising a nucleic acid sequence encoding an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 2, 4, and 6;
- and
- ii) a cultured host cell;
- and
- b) introducing said vector into said host cell under conditions such that said host cell expresses said amino acid sequences, wherein said sequence encodes a calcium channel protein.
A method for coexpressing a plurality of calcium channel proteins, comprising:
- a) providing:
- i) one or more recombinant expression vectors comprising one or more nucleic acid sequences encoding amino acid sequences selected from the group consisting of SEQ ID NOs:
- 2, 4, and 6;
- and
- ii) a cultured host cell;
- and
- b) introducing said vectors into said host cell under conditions such that said host cell coexpresses said one or more amino acid sequences, wherein said amino acid sequences encode calcium channel proteins.
Dependencies are inferred from the claim text.