Plant prohibition genes and their use
AssigneePioneer Hi-Bred International, Inc.
InventorsWilliam J. Gordon-Kamm, Keith S. Lowe, Ramgopal Nadimpalli, Carl R. Simmons
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Abstract
Compositions and methods for modulating cell cycle and cell proliferation are provided. Additionally the compositions find use in enhancing disease resistance and increasing transformation efficiency in plants. The method involves transforming a plant with a sense or antisense prohibitin sequence. The prohibitin sequence acts to regulate cell division in the plant cell. Transformed plants, plant cells, tissues, and seed are also provided having enhanced disease resistance.
Claims24
An isolated nucleic acid molecule comprising a nucleotide sequence set forth in SEQ ID NO:
- 1.
An isolated nucleic acid molecule comprising a nucleotide sequence set forth in SEQ ID NO:
- 3.
An isolated nucleic acid molecule comprising a nucleotide sequence set forth in SEQ NO:
- 5.
An isolated nucleic acid molecule comprising a nucleotide sequence set forth in SEQ ID NO:
- 7.
An isolated nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of:
- a) a nucleotide sequence comprising a cDNA sequence deposited as Patent Deposit Nos. 98867, 98868, 98869, or 98870;
- b) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence set forth in SEQ ID NO:
- 2, 4, 6, or 8;
- and,
- c) a nucleotide sequence comprising an antisense sequence fully complementary to the sequence set forth in SEQ ID NO:
- 1, 3, 5, or 7.
A plant having stably incorporated into its genome a nucleotide sequence operably linked to a heterologous promoter active in said plant, said nucleotide sequence selected from the group consisting of:
- a) a nucleotide sequence set forth in SEQ ID NO:
- 1, 3, 5, or 7;
- b) a nucleotide sequence comprising a cDNA sequence deposited as Patent Deposit Nos. 98867, 98868, 98869, or 98870;
- and,
- c) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence set forth in SEQ ID NO:
- 2, 4, 6 or 8.
An plant cell having stably incorporated into its genome a nucleotide sequence operably linked to a heterologous promoter active in said plant cell, said nucleotide sequence selected from the group consisting of:
- a) a nucleotide sequence set forth in SEQ ID NO:
- 1, 3, 5, or 7;
- b) a nucleotide sequence comprising a cDNA sequence deposited as Patent Deposit Nos. 98867, 98868, 98869, or 98870;
- and,
- c) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence set forth in SEQ ID NO:
- 2, 4, 6, or 8.
A method of modulating the level of a prohibitin polypeptide in a plant comprising stably incorporating into the genome of said plant a nucleotide sequence operably linked to a heterologous promoter active in said plant, said nucleotide sequence selected from the group consisting of:
- a) a nucleotide sequence set forth in SEQ ID NO:
- 1, 3, 5, or 7;
- b) a nucleotide sequence comprising a cDNA sequence deposited as Patent Deposit Nos. 98867, 98868, 98869, or 98870;
- c) a nucleotide sequence encoding a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:
- 2, 4, 6 or 8 and
- d) a nucleotide sequence comprising an antisense sequence fully complementary to the sequence set forth in SEQ ID NO:
- 1, 3, 5, or 7.
Dependencies are inferred from the claim text.