Back to results
US 6,441,150 B1Granted
Compounds for the Synthesis of Nucleotide or oligonucleotide phosphoramidites
AssigneeIsis Pharmaceuticals, Inc.
InventorsRichard H. Griffey, Douglas L. Cole, Vasulinga Ravikumar
Life of this patent
Estimating the term…
Abstract
This invention presents novel methods for recovery of phosphoramidites from the waste products of oligonucleotide synthesis. The methods include reacting a tribromophenoxydichlorophosphorane with an H-phosphonate in the presence of an amine.
Claims24
A compound of formula:
- wherein:
- Z is an intersugar linkage;
- R1 is a hydroxyl protecting group;
- R2 is H, OH, O-alkyl, O-alkylamino, O-alkylalkoxy, a polyether of formula (O-alkyl)m where m is 1 to about 10, or a protected hydroxyl group;
- R3 is 4-cyano-2-butenyl or diphenylmethylsilylethyl;
- B is a nucleobase;
- and
- n is 1 to about 100.
5 more elements
A compound of formula:
- wherein:
- Z is an intersugar linkage;
- R1 is a hydroxyl protecting group;
- R2 is H, OH, O-alkyl, O-alkylamino, O-alkylalkoxy, a polyether of formula (O-alkyl)m where m is 1 to about 10, or a protected hydroxyl group;
- R3 is 4-cyano-2-butenyl or diphenylmethylsilylethyl;
- B is a nucleobase;
- and
- n is 1 to about 100.
5 more elements
The compound of claim wherein R3 is cyanoethyl.
Dependencies are inferred from the claim text.