Method for recovering proteins from the interstitial fluid of plant tissues
AssigneeLarge Scale Biology Corporation
InventorsThomas H. Turpen, Stephen J. Garger, Michael J. McCulloch, Terri I. Cameron, Michelle L. Samonek-Potter, R. Barry Holtz
Life of this patent
Estimating the term…
Abstract
A method for extracting proteins from the intercellular space of plants is provided. The method is applicable to the large scale isolation of many active proteins of interest synthesized by plant cells. The method may be used commercially to recover recombinantly produced proteins from plant hosts thereby making the large scale use of plants as sources for recombinant protein production feasible.
Claims60
A method for recovery of a concentrated and active protein of interest from the interstitial fluid of a plant tissue comprising the steps of:
- (a) infiltrating a plant tissue with a buffer solution by a continuous delivery of said buffer solution to said plant tissue by means of a rotary vacuum infiltration tank;
- (b) subjecting the plant tissue and buffer solution to a substantially vacuum environment;
- (c) removing the plant tissue from the buffer solution;
- (d) centrifuging the plant tissue to remove interstitial fluid;
- and
- (e) concentrating the protein of interest from the interstitial fluid.
A method for recovery of a biomolecule of interest selected from the group consisting of:
- lipids, carbohydrates, lipoproteins, polysaccharides, sugars, fatty acids, nucleic acids and polynucleotides, from the interstitial fluid of a plant tissue, said method comprising the steps of:
- (a) infiltrating a plant tissue with a buffer solution by a continuous delivery of said buffer solution to said plant tissue by means of a rotary vacuum infiltration tank;
- (b) subjecting the plant tissue and buffer solution to a substantially vacuum environment;
- (c) removing the plant tissue from the buffer solution;
- (d) centrifuging the plant tissue to remove interstitial fluid;
- (e) concentrating the biomolecule of interest from the interstitial fluid.
Dependencies are inferred from the claim text.