Transgenically produced Antithrombin III
AssigneeGenzyme Transgenics Corporation
InventorsPaul DiTullio, Harry Meade, Edward S. Cole
Life of this patent
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Abstract
This invention relates to transgenically produced human Antithrombin III (tgATIII). The human ATIII produced by the transgenic process of the present invention has a monosaccharide composition which comprises N-acetylgalactosamine (GaINAc) along with fucose, N-acetylglucosamine, galactose, mannose, and N-acetylneuraminic acid/N-glycolyneuraminic acid. The monosaccharide composition differs with that of plasma derived ATIII (phATIII). It has been found that tgATIII has an increased clearance rate when compared to phATIII.
Claims20
Mammary gland produced antithrombin III having a monosaccharide composition which comprises GalNAc.
Mammary gland produced antithrombin III having a monosaccharide composition which comprises fucose.
Mammary gland produced antithrombin III having a monosaccharide composition which comprises a higher level of mannose than plasma derived antithrombin III.
A method for producing antithrombin III in mammalian milk, comprising:
- a. providing a transgenic mammal that expresses in its mammary tissue a transgene which encodes a human antithrombin III with a monosaccharide composition which comprises GalNAc, wherein said human antithrombin III is secreted into the milk of the mammal;
- and
- b. collecting milk from the transgenic animal which contains the human antithrombin III;
- c. to thereby obtain human antithrombin III with a monosaccharide composition which includes GalNAc.
A glycosylated human antithrombin III which is produced in mammary gland of a non-human transgenic mammal, wherein the antithrombin III comprises a monosaccharide composition which comprises GalNAc.
A glycosylated human antithrombin III which is produced in the mammary gland of a non-human transgenic mammal, wherein the antithrombin III comprises a monosaccharide composition which comprises fucose.
A glycosylated human antithrombin III which is produced in the mammary gland of a non-human transgenic mammal, wherein the antithrombin III comprises a monosaccharide composition which comprises a higher level of mannose than plasma derived antithrombin III.
A method for producing antithrombin III in mammalian milk, comprising:
- providing a transgenic mammal that expresses in its mammary tissue a transgene which encodes a human antithrombin III with a monosaccharide composition which comprises fucose, wherein said human antithrombin III is secreted into the milk of the mammal;
- and
- collecting milk from the transgenic animal which contains the human antithrombin III
- to thereby obtain human antithrombin III with a monosaccharide composition which includes fucose.
A method for producing antithrombin III in mammalian milk, comprising:
- providing a transgenic mammal that expresses in its mammary tissue a transgene which encodes a human antithrombin III having a monosaccharide composition which comprises a higher level of mannose than plasma derived antithrombin III, wherein said human antithrombin III is secreted into the milk,of the mammal;
- and
- collecting milk from the transgenic animal which contains the human antithrombin III
- to thereby obtain human antithrombin III having a monosaccharide composition which includes a higher level of mannose than plasma derived antithrombin III.
Dependencies are inferred from the claim text.