Synthetic peptides, antibodies against them and their use
AssigneeDade Behring Marburg GmbH
InventorsMichael Kraus, Werner Stüber
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Abstract
The present invention is directed to the generation of antibodies which preferentially bind to fibrinogen fragments E1, E2 and E3, but exhibit little or no cross-reactivity against fibrin monomer and fibrinogen. Thus, the invention provides synthetic peptides containing defined amino acid sequences corresponding to the carboxy terminal regions of the E fragments which arise as a result of plasmin cleavage of fibrin and fibrinogen. The synthetic peptides may be synthesized chemically, or through genetic manipulations, and may contain additional amino acid sequences which are not contiguous with the defined E fragment sequences.
Claims11
A method of producing antibodies comprising:
- 1)immunizing an animal with an immunogenic composition comprising a synthetic peptide comprising an amino acid sequence corresponding to the carboxy terminal regions of an E fragment arising as a result of plasmin cleavage of fibrin and fibrinogen,
- 2) isolating antibodies raised against said peptide, and
- 3) purifying or immunoabsorbing said antibodies,
- wherein the amino acid sequence corresponding to the carboxy terminal regions of an E fragment arising as a result of plasmin cleavage of fibrin and fibrinogen is selected from the group consisting of:
- a) Leu-Phe-Glu-Tyr-Gln-Lys-OH, (SEQ ID NO:1),
- b) Tyr-Met-Tyr-Leu-Leu-Lys-OH, (SEQ ID NO:2),
- c) Val-Lys-Glu-Leu-Ile-Lys-OH, (SEQ ID NO:3), and
- d) His-Gln-Val-Glu-Asn-Lys-OH (SEQ ID NO:4).
Dependencies are inferred from the claim text.