Inhibitors of glycogen synthase kinase-3 and methods for identifying and using the same
AssigneesThe Trustees of the University of Pennsylvania, President and Fellows of Harvard University
InventorsPeter S. Klein, Douglas Melton
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Abstract
A method of identifying inhibitors of glycogen synthase kinase-3 is provided. The method comprises providing a mixture comprising GSK-3, a phosphate source, and a GSK-3 substrate, incubating the mixture in the presence or absence of a test compound, and assessing the activity of GSK-3 in the mixture. A reduction of GSK-3 activity following incubation of the mixture in the presence of the test compound is an indication that the test compound is an inhibitor of GSK-3.
Claims1
A method of treating a GSK-3 related disorder other than Alzheimer's disease in an animal comprising administering to said animal a GSK-3 inhibitor suspended in a pharmaceutically acceptable carrier, wherein said GSK-3 inhibitor is not lithium and is identified by:
- (a) providing a mixture comprising GSK-3, a source of phosphate, a GSK-3 substrate, and a GSK-3 assay buffer;
- (b) incubating said mixture in the presence or absence of a test compound;
- (c) measuring the level of phosphorylation of said GSK-3 substrate;
- and
- (d) determining whether the level of phosphorylation of said GSK-3 substrate is lower in the presence of said test compound than in the absence of said test compound, and further wherein the GSK-3 related disorder is selected from the group consisting of bipolar disorder, mania, and leukopenia.
Dependencies are inferred from the claim text.