Process and device for carrying out fluorescence immunoassays

A method for carrying out fluorescence immunoassays comprising the steps of:

• coupling light from at least one light source (16, 17) into a light waveguide via a surface at one end of the light waveguide (1), and coating the light waveguide (1) with a chemical or biochemical component and introducing the coating into a measuring chamber (9);
• disposing the measuring chamber with the light waveguide within a cylinder having a piston therein, wherein at least one of said cylinder and piston reciprocally moves relative to each other;
• conducting sample liquid through ee measuring chamber (9) along the surface of the light waveguide (1) into a sample collecting chamber (10), coupling into the light waveguide simultaneously with excitation of fluorescence with light from the light source (16, 17,) and directing the fluorescent light decoupled from the light waveguide (1) onto an optical detector (22) which measures intensity of the fluorescent light, so that on throughflow of the sample liquid, binding of a fluoresce-labeled component of a general receptor-ligand system to a partner immobilized on the surface of the light waveguide (1) is measured with time resolution, wherein said piston has a piston face and the cylinder has an inlet opening with a surface and at least one of the piston face (23), an inner surface of the cylinder (7), and the surface of the inlet opening 11 is covered with a chemical or biochemical component before carrying out the fluorescence immunoassay, wherein on the light waveguide is formed a surface having a closeable opening for flow of the sample liquid through the measuring chamber (9), the closable opening (3) being at least partially released.

A method for carrying out fluorescence immunoassays comprising the steps of:

• coupling light from at least one light source (16, 17) into a light waveguide via a surface at one end of the light waveguide (1), and coating the light waveguide (1) with a chemical or biochemical component and introducing the coating into a measuring chamber (9);
• disposing the measuring chamber with the light waveguide within a cylinder having a piston therein, said cylinder and piston reciprocally movable relative to each other;
• conducting sample liquid through the measuring chamber (9) along the surface of the light waveguide (1) into a sample collecting chamber (10), coupling into the light waveguide simultaneously with excitation of fluorescence with light from the light source (16, 17,) and directing the fluorescent light decoupled from the light waveguides (1) onto an optical detector (22) which measures intensity of the fluorescent light, so that on throughflow of the sample liquid, binding of a fluorescence-labeled component of a general receptor-ligand system to a partner immobilized on the surface of the light waveguide (1) is measured with time resolution, wherein said piston has a piston face and the cylinder has an inlet opening with a surface and at least one of the piston face (23), an inner surface of the cylinder (7), and the surface of the inlet opening (11) is covered with a chemical or biochemical component before carrying out the fluorescence immunoassay, wherein the cylinder has a longitudinal axis and, when the inlet opening at the cylinder (7) is closed, when the opening (3) is closed or in a case of a surface (2) without an opening (3), with the piston (6) fixed and with the surface (2) with light waveguide (1) fixed, the cylinder (7) reciprocally moves parallel to the longitudinal axis of the cylinder, so that the sample liquid flows through the measuring chamber (9) according to the direction of movement of the cylinder (7).