Stabilization of biological fluids by addition of sterol esters
AssigneeDade Behring Marburg GmbH
InventorHarald Althaus
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Abstract
The present invention relates to the use of sterol esters for the long-term stabilization of biological fluids, in particular even those which are obtained by lyophilization and subsequent reconstitution.
Claims21
A method of producing a stabilized biological fluid, comprising adding to a biological fluid at least one sterol ester of formula I:
- R1—O—(O)C—R2—C(O)—O—(CH2—CH2—O)n—CH2—CH2—OH Formula I
- wherein
- n=1-200;
- R1 =a sterol;
- and
- R2 is an aliphatic or aromatic ring having from 4 to 8 carbon atoms, wherein one or more of the ring carbon atoms is optionally replaced by N, S, or O, or is a linear aliphatic carbon chain having from 0 to 12 carbon atoms, or is a branched aliphatic carbon chain having from 0 to 12 carbon atoms, wherein 0 carbon atoms represents a bond;
- wherein the sterol ester is added to the biological fluid in a concentration of from 0.05% to 5% by weight;
- and
- wherein for a period of between 12 and 18 months after addition of the sterol ester to the biological fluid, the biological fluid has a turbidity measured at 546 nanometers that is increased by no more than 300% compared with the turbidity of the biological fluid before addition of the sterol ester.
A composition of matter, comprising
- a natural or artificial matrix;
- at least one substance to be analyzed;
- and
- at least one sterol ester of Formula I:
- R1—O—(O)C—R2—C(O)—O—(CH2—CH2—O)n—CH2—CH2—OH Formula I
- in a concentration of 0.05 to 5% by weight, wherein
- n=1-200;
- R1=a sterol;
- and
- R2 is an aliphatic or aromatic ring having from 4 to 8 carbon atoms, wherein one or more of the ring carbon atoms is optionally replaced by N, S, or O, or is a linear aliphatic carbon chain having from 0 to 12 carbon atoms, or is a branched aliphatic carbon chain having from 0 to 12 carbon atoms, wherein 0 carbon atoms represents a bond;
- and
- wherein for a period of between 12 and 18 months after preparing the composition, the composition has a turbidity measured at 546 nanometers that is increased by no more than 300% compared with a composition that lacks the at least one sterol ester but is otherwise identical.
The composition as claimed in claimed 13, wherein for a period of between 12 and 18 months after addition of the sterol ester to the composition, the composition has a turbidity measured at 546 nanometers that is increased by no more than 300% compared with the turbidity of-the composition immediately after addition of the sterol ester.
A method of producing a stabilized artificial matrix, comprising adding to an artificial matrix at least one sterol ester of formula I:
- R1—O—(O)C—R2—C(O)—O—(CH2—CH2—O)n—CH2—CH2—OH Formula I
- wherein
- n=1-200;
- R1=a sterol;
- and
- R2 is an aliphatic or aromatic ring having from 4 to 8 carbon atoms, wherein one or more of the ring carbon atoms is optionally replaced by N, S, or O, or is a linear aliphatic carbon chain having from 0 to 12 carbon atoms, or is a branched aliphatic carbon chain having from 0 to 12 carbon atoms, wherein 0 carbon atoms represents a bond;
- wherein the sterol ester is added to the artificial matrix in a concentration of from 0.05% to 5% by weight;
- and
- wherein for a period of between 12 and 18 months after addition of the sterol ester to the artificial matrix, the artificial matrix has a turbidity measured at 546 nanometers that is increased by no more than 300% compared with the turbidity of the artificial matrix before addition of the sterol ester.
Dependencies are inferred from the claim text.