Methods of using polynucleotide compositions
AssigneeSupratek Pharma Inc.
InventorsAlexander V. Kabanov, Valery Y. Alakov, Serguie Vinogradov
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Abstract
Compositions for stabilizing polynucleic acids and increasing the ability of polynucleic acids to cross cell membranes and act in the interior of a cell. In one aspect, the invention provides a polynucleotide complex between a polynucleotide and certain polyether block copolymers. The polynucleotide complex can further include a polycationic polymer, as well as suitable targeting molecules and surfactants. The invention also provides a polynucleotide complex between a polynucleotide and a block copolymer comprising a polyether block and a polycation block.
Claims13
A method for delivering a polynucleotide to a cell comprising administering to the cell an effective amount of a composition comprising noncovalent complex of said polynucleotide and a polymer comprising a plurality of covalently bound segments comprising:
- (a) at least one polycationic homopolymer or copolymer having at least three cationic sites, or a protonated or quaternary form thereof, said polycationic homopolymer or copolymer comprising at least one member selected from the group consisting of
- (i) a tertiary amino monomer of the formula:
- and
- (ii) a secondary amino monomer of the formula:
- in which:
- each of R1, R5, R6, and R8, independently of the others, is hydrogen, alkyl of 2 to 8 carbon atoms, an A monomer, or a B monomer;
- each of R2, R3, and R7, taken independently of the others, is the same or different straight or branched chain alkanediyl group of the formula:
- in which z has a value of from 2 to 8;
- and
- R4 is hydrogen;
- and
- (b) at least one water-soluble nonionic polymer segment.
Dependencies are inferred from the claim text.