Altering the properties of cells or of particles with membranes derived from cells by means of lipid-modified proteinaceous molecules
AssigneeU-BiSys B.V.
InventorsTom Logtenberg, Cornelis Adriaan De Kruif
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Abstract
A processs for altering the properties of a cell or cell membrane involves the contacting of the cell or cellular membrane with a lipid-modified protein under conditions wherein the lipid portions anchor themselves to the cellar membrane and position the protein portion of the molecule so that it imparts to the cell or cell membrane one or more new characteristics resulting from the introduction of the protein. Recombinant and cell-free methods for synthesis of the lipid-modified protein are taught as are kits for altering the properties of cell and/or cell membranes.
Claims21
A process for altering the properties of a target cell and/or a target cellular membrane through the direct introduction of a first heterologous protein to the target cell and/or target cellular membrane surface comprising contacting said target cell and/or target cellular membrane with an effective amount of a lipid-modified protein,
- wherein said lipid-modified protein is enzymatically synthesized to contain bound lipid either in a cell or in a cell-free system from a fusion protein that has at least one first portion derived from said first heterologous protein and at least one second portion comprising a lipidation signal derived from a second protein, wherein said second protein is a lipoprotein,
- wherein the effective amount is sufficient to impart to the target cell and/or target cell membrane surface an additional characteristic resulting from the first heterologous portion, and
- the lipid bound to the lipid-modified protein attaches the lipid-modified protein directly to the target cell and/or target cellular membrane.
A process for altering the properties of a target cell and/or a target cellular membrane through the direct introduction of a first heterologous protein to the target cell and/or target cellular membrane surface comprising contacting said target cell and/or target cellular membrane with an effective amount of a lipid-modified protein,
- wherein said lipid-modified protein is enzymatically synthesized to contain bound lipid either in a cell or in a cell-free system from a fusion protein that has at least one first portion derived from said first heterologous protein and at least one second portion comprising a lipidation signal derived from a second protein,
- wherein the effective amount is sufficient to impart to the target cell and/or target cell membrane surface an additional characteristic resulting from the first heterologous portion, and
- the lipid bound to the lipid-modified protein attaches the lipid-modified protein directly to the target cell and/or target cellular membrane, and
- wherein at least part of said first portion is derived from a single chain variable antibody fragment.
A process for altering the properties of a target cell and/or a target cellular membrane through the direct introduction of a first heterologous protein to the target cell and/or target cellular membrane surface comprising contacting said target cell and/or target cellular membrane with an effective amount of a lipid-modified protein,
- wherein said lipid-modified protein is enzymatically synthesized to contain bound lipid either in a cell or in a cell-free system from a fusion protein that has at least one first portion derived from said first heterologous protein and at least one second portion comprising a lipidation signal derived from a second protein,
- wherein the effective amount is sufficient to impart to the target cell and/or target cell membrane surface an additional characteristic resulting from the first heterologous portion, and
- the lipid bound to the lipid-modified protein attaches the lipid-modified protein directly to the target cell and/or target cellular membrane, and
- wherein said lipid-modified protein has a detection tag.
Dependencies are inferred from the claim text.