Retroviral hybrid vectors pseudotyped with LCMV
AssigneeHeinrich-Pette-Institut
InventorsMeike-Dorothee Von Laer, Winfried Beyer
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Abstract
The present invention relates in general to the pseudotyping of retroviruses with lymphocytic choriomeningitis virus. In particular, the invention relates to pseudotyping in MLV packaging cells which are optionally env-deleted, or in packaging cells derived from lentiviruses. Preferably, pseudotyping takes place by infection with LCMV or a preferably env-deleted mutant, or by transfection with an expression plasmid containing the gp gene of LCMV or a part thereof and optionally, in addition, the np, 1 and/or the z gene of LCMV. The invention also relates to the use of such pseudotypes for the infection of cells, particularly the use in gene therapy.
Claims19
A retroviral packaging cell, which contains gag and pol genes of a retroviral vector and a nucleic acid sequence coding for the glycoproteins GP-1 and GP-2 of lymphocytic choriomeningitis virus (LCMV), wherein the gag and pol genes and said glycoproteins are expressed in said retroviral packaging cell.
The retroviral packaging cell, which expresses pseudotyped virions which contain LCMV glycoprotein inserted in the coat of said pseudotyped virions.
Dependencies are inferred from the claim text.