Proteinase inhibitor, precursor thereof and genetic sequences encoding same
AssigneeThe University of Melbourne
InventorsMarilyn Anne Anderson, Angela Hilary Atkinson, Robyn Louise Heath, Adrienne Elizabeth Clarke
Life of this patent
Estimating the term…
Abstract
The present invention relates generally to proteinase inhibitors, a precursor thereof and to genetic sequences encoding same. More particularly, the present invention relates to a nucleic acid molecule comprising a sequence of nucleotides which encodes or is complementary to a sequence which encodes a type II serine proteinase inhibitor (PI) precursor from a plant wherein said precursor comprises at least three PI monomers and wherein at least one of said monomers has a chymotrypsin specific site and at least one other of said monomers has a trypsin specific site.
Claims4
A genetic construct comprising a nucleotide sequence which encodes or is complementary to a sequence which encodes a type II serine proteinase inhibitor (PI) precursor from a plant or monomer of said PI precursor, wherein said nucleotide sequence is a sequence as set forth in SEQ ID NO:
- 1 or a sequence which hybridizes to the complement sequence of SEQ ID NO:
- 1 under the conditions of at least one of 4×SSC at room temperature, 2×SSC at room temperature, 1×SSC at 40° C., 2×SSC with 0.1% w/v SDS at 68° C., or 0.2×SSC with 1% w/v SDS at 68° C., wherein said precursor comprises at least three PI monomers and wherein at least one of said monomers has a chymotrypsin specific site and at least one of said other monomers has a trypsin specific site, and wherein said genetic construct further comprises expression means to permit expression of said nucleotide sequence, replication means to permit replication in a plant cell, or integration means to permit stable integration of said nucleotide sequence into a plant cell genome.
A transgenic plant carrying a genetic construct, said genetic construct comprising a nucleotide sequence which encodes or is complementary to a sequence which encodes a type II serine proteinase inhibitor (PI) precursor from a plant or monomer of said PI precursor, wherein said nucleotide sequence is a sequence as set forth in SEQ ID NO:
- 1 or a sequence which hybridizes to the complement sequence of SEQ ID NO:
- 1 under the conditions of at least one of 4×SSC at room temperature, 2×SSC at room temperature, 1×SSC at 40° C., 2×SSC with 0.1% w/v SDS at 68° C., or 0.2×SSC with 1% w/v SDS at 68° C., wherein said precursor comprises at least three PI monomers and wherein at least one of said monomers has a chymotrypsin specific site and at least one other of said monomers has a trypsin specific site.
Dependencies are inferred from the claim text.