Integrated fluid manipulation cartridge

A device for use with an ultrasonic transducer to separate an analyte from a fluid sample containing cells, spores, or microorganisms, the device comprising a cartridge having:

• a) a lysing chamber for lysing the cells, spores, or microorganisms to produce a lysate containing the analyte, wherein the lysing chamber contains a membrane or filter for capturing the cells, spores, or microorganisms as the sample flows through the lysing chamber, and wherein the lysing chamber is defined by at least one wall for contacting the transducer to effect the transfer of ultrasonic energy to the lysing chamber;
• b) an extraction chamber for receiving the lysate, the extraction chamber containing at least one solid support for capturing the analyte in the lysate as the lysate flows through the extraction chamber;
• c) an elution flow path passing through the extraction chamber for eluting the captured analyte from the extraction chamber;
• d) at least one waste chamber for receiving waste fluid from the lysing and extraction chambers;
• and
• e) flow control means for:
• i) directing the sample to flow through the lysing chamber and into the at least one waste chamber, thereby capturing the sample components to be lysed with the membrane or filter in the lysing chamber;
• ii) directing the lysate to flow from the lysing chamber through the extraction chamber and into the at least one waste chamber, thereby capturing the analyte in the lysate with the at least one solid support in the extraction chamber;
• and
• iii) directing an elution fluid to flow through the extraction chamber and along the elution flow path, thereby eluting the analyte from the extraction chamber.

A device for separating nucleic acid from a fluid sample and for holding the nucleic acid for an amplification reaction, the device comprising:

• a) a cartridge having:
• i) a first flow path including:
• an extraction chamber containing at least one solid support for capturing the nucleic acid;
• and
• a waste chamber, downstream of the extraction chamber, for receiving waste fluid;
• ii) a second flow path for eluting the captured nucleic acid from the extraction chamber, wherein the second flow path passes through the extraction chamber and diverges from the first flow path after passing through the extraction chamber;
• and
• iii) at least one flow controller for directing the waste fluid into the waste chamber and for directing the eluted nucleic acid into the second flow path;
• and
• b) a reaction vessel coupled to the cartridge for receiving the eluted nucleic acid, the reaction vessel having a reaction chamber for holding the nucleic acid for the amplification reaction, and the reaction vessel being coupled to the cartridge such that the vessel protrudes from the body of the cartridge.

A device for separating nucleic acid from a fluid sample and for holding the nucleic acid for an amplification reaction, the device comprising a cartridge having:

• a) a first flow path including:
• i) an extraction chamber containing at least one solid support for capturing the nucleic acid;
• and
• ii) a waste chamber, downstream of the extraction chamber, for receiving waste fluid;
• b) a second flow path for eluting the captured nucleic acid from the extraction chamber, wherein the second flow path passes through the extraction chamber and diverges from the first flow path after passing through the extraction chamber;
• c) at least one flow controller for directing the waste fluid into the waste chamber and for directing the eluted nucleic acid into the second flow path;
• and
• d) a reaction chamber for receiving the eluted nucleic acid via the second flow path and for holding the nucleic acid for the amplification reaction, wherein the portion of the cartridge defining the reaction chamber protrudes from the rest of the cartridge body.