Method and device for preparing samples for detecting a nucleotide sequence
Assigneenovember Aktiengesellschaft Gesellschaft fur Molekulare Medizin
InventorWolf Bertling
Life of this patent
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Abstract
A method for preparing samples for detecting a nucleotide sequence by polymerase chain reaction (PCR) according to which a) an analysis solution is filled into at least one cavity (2) provided for in a support (1); b) a lid (3) configured complementary to the shape of the cavity (2) is placed onto the support (1) in such a way that the analysis solution is pushed at least partly into a gap (S) formed between the cavity (2) and the lid (3); and c) the gap (S) is sealed by means of at least one seal (5, 12) provided for near an opening in the cavity (2).
Claims13
A method for preparing samples for detecting a nucleotide sequence by means of polymerase chain reaction (PCR) where the sample is contacted with an analysis solution, comprising the steps of:
- a) providing a support (1) defining at least one cavity, and filling the analysis solution into said at least one cavity (2),
- b) placing a lid (3), which is configured complementary to a shape of the cavity (2), onto the support (1) such that at least some of the analysis solution is displaced into a gap (G) formed between the cavity (2) and the lid (3), a primer being bound to an internal face (I) of the lid (3), which extends into the cavity (2),
- c) sealing the gap (G) by means of at least one seal (5, 12) provided near an opening of the cavity (2) and
- d) shifting the nucleotide sequence toward the primer under the influence of an electrical field and binding the nucleotide sequence to the primer.
Dependencies are inferred from the claim text.