Arrays with modified oligonucleotide and polynucleotide compositions
AssigneeOligos Etc. Inc.
InventorRoderic M. K. Dale
Life of this patent
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Abstract
Binding units such as avidin or biotin are placed on tightly defined areas of a solid planar support surface. Polymer sequences such as oligonucleotides are synthesized with a complementary binding unit attached, preferably via a linker moiety. The polymer/linker/binding units are then placed on the binding units on the support surface and an array is formed.
Claims16
An array comprising a plurality of modified oligonucleotide compositions stably associated with a surface of a support, comprising:
- a support comprised of a plurality of distinct areas on a surface of the support, said areas having a plurality of a first binding unit bound to the surface;
- and
- a plurality of different modified oligonucleotide compositions wherein each composition is characterized by an oligonucleotide backbone structure modified from that of a naturally occurring nucleotide polymer, wherein oligonucleotides of the compositions comprise a second binding unit which forms a non-covalent bond with said first binding unit and a blocking chemical modification at or near at least one end of each oligonucleotide;
- wherein each different modified oligonucleotide composition is stably associated with a different distinct area of the support, and further wherein modified oligonucleotides of each composition are characterized by a pH stability of at least one hour at 37° C. at a pH range of about 0.5 to about 6.0 and a nuclease resistance of at least twice that of a naturally occurring oligonucleotide having the same sequence and number of bases.
A method of synthesizing an array comprising a plurality of modified oligonucleotide compositions stably associated with a surface of a support, said method comprising:
- synthesizing a support comprising a plurality of distinct areas having a plurality of a first binding unit bound to the surface;
- synthesizing a plurality of different modified oligonucleotide compositions wherein each composition comprises:
- an oligonucleotide backbone structure modified from that of a naturally occurring nucleotide polymer, and
- a second binding unit which forms a non-covalent bond with said first binding unit and a blocking chemical modification at or near at least one end of each oligonucleotide;
- wherein the modified oligonucleotides of each composition are characterized by a pH stability of at least one hour at 37° C. at a pH range of about 0.5 to about 6.0 and a nuclease resistance of at least twice that of a naturally occurring oligonucleotide having the same sequence and number of bases;
- and
- stably associating each modified oligonucleotide composition to a distinct area of said support.
Dependencies are inferred from the claim text.