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US 6,440,720 B1Granted
Pichia methanolica glyceraldehyde-3-phosphate dehydrogenase 2 promoter and terminator
AssigneeZymoGenetics, Inc.
InventorChristopher K. Raymond
Life of this patent
Estimating the term…
Abstract
Transcription promoter and terminator sequences from the Pichia methanolica glyceraldehyde-3-phosphate dehydrogenase 2 gene (GAP2 gene) are disclosed. The sequences are useful within DNA constructs for the production of proteins of interest in cultured P. methanolica cells. Within the expression vectors, a GAP2 promoter and/or a GAP2 terminator is operably linked to a DNA segment encoding the protein of interest.
Claims20
A DNA construct comprising the following operably linked elements:
- a first DNA segment comprising a Pichia methanolica gene transcription promoter;
- a second DNA segment encoding a protein of interest other than a Pichia methanolica protein;
- and
- a third DNA segment comprising nucleotides 2095 to 2145 of SEQ ID NO:2.
1 more element
Dependencies are inferred from the claim text.