Method for producing non-infectious recombinant picornavirus particles
Assigneenovember Aktiengesellschaft Gesellschaft fur Molekulare Medizin
InventorChristian Probst
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Abstract
The invention relates to a process for recombinantly preparing picornavirus particles, in particular hepatitis A virus particles, their precursors and particles which are derived therefrom, with a structural protein precursor molecule (P1-2A or P1) and the corresponding P3 region (3ABCD) being coexpressed in cis or in trans.
Claims16
A method for recombinantly producing non-infectious Hepatitis A virus particles comprising expressing a nucleic acid encoding a Hepatitis A virus structural protein precursor molecule and a nucleic acid encoding a Hepatitis A virus P3 region in a cell, wherein said cell does not express Hepatitis A virus proteins 2B and 2C.
A kit for producing recombinant non-infectious Hepatitis A virus particles, said kit comprising:
- a) a nucleic acid encoding a Hepatitis A virus structural protein precursor molecule;
- and
- b) a nucleic acid encoding a Hepatitis A virus P3 region.
Dependencies are inferred from the claim text.