Fermentation of clavulanic acid at a controlled level of ammonia
AssigneeDsm N.V.
InventorsGesina Visser-Luirink, Wilhelmus Theodorus Antonius De Laat, Jeroen Martijn Klop
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Abstract
A method for the production of clavulanic acid by the fermentation of a clavulanic acid producing microorganism at a controlled level of at least 50 mg/l ammonia has been provided for. Under the application of these reaction conditions surprisingly high production levels of clavulanic acid have been obtained.
Claims6
A method for the production of clavulanic acid comprising regulating the concentration of ammonium ion during fermentation of a clavulanic acid producing microorganisms on a suitable medium comprising carbon and nitrogen sources wherein the concentration of ammonium ion is maintained from 100 mg/l to 2,500 mg/l by regulating the rate of ammonium ion addition and/or regulating the addition of at least one titrant for ammonium ion.
- 2.The method according to claim 1, wherein the ammonium ion addition is the addition of ammonium sulfate or urea.
The method of claim 1 further comprising recovering the clavulanic acid.
- 7.The method of claim 6, further comprising converting the recovered clavulanic acid into an amine salt thereof by adding the corresponding amine salt forming compound thereto and reacting said amine clavulanate with a non-toxic, pharmaceutically acceptable salt, and
- purifying the salt of clavulanic acid.
Dependencies are inferred from the claim text.