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US 6,440,703 B1Granted
Enzymatic synthesis of gangliosides
AssigneeNeose Technologies, Inc.
InventorShawn DeFrees
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Abstract
This invention provides methods for practical in vitro synthesis of gangliosides and other glycolipids. The synthetic methods typically involve enzymatic synthesis, or a combination of enzymatic and chemical synthesis. One or more of the enzymatic steps is preferably carried out in the presence of an organic solvent.
Claims18
A method of enzymatically synthesizing a glycosphingod from a sphingoid, the method comprising:
- (a) removing the fatty acid from the sphingoid;
- (b) contacting the product from step (a) with a glycosyltransferase reaction mixture comprising:
- i) at least one glycosyltransferase,
- ii) a corresponding nucleotide sugar for each glycosyltransferase,
- iii) other reactants required for glycosyltransferase activity;
- and
- iv) an organic solvent for a sufficient time and under appropriate conditions to transfer a sugar from said nucleotide sugar to the product from step (a), thereby forming said glycosphingoid.
4 more elements
Dependencies are inferred from the claim text.