Transferrin receptor genes of Moraxella
AssigneeAventis Pasteur Limited
InventorsLisa E. Myers, Anthony B. Schryvers, Robin E. Harkness, Sheena M. Loosmore, Run-Pan Du, Yan-Ping Yang, Michel H. Klein
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Abstract
Purified and isolated nucleic acid molecules are provided which encode transferrin receptor proteins of Moraxella, such as M. catarrhalis or a fragment or an analog of the transferrin receptor protein. The nucleic acid sequence may be used to produce recombinant transferrin receptor proteins Tbp1 and Tbp2 of the strain of Moraxella free of other proteins of the Moraxella strain for purposes of diagnostics and medical treatment. Furthermore, the nucleic acid molecule may be used in the diagnosis of infection.
Claims13
A purified and isolated nucleic acid molecule consisting of a DNA sequence selected from the group consisting of:
- (a) a DNA sequence as set out in FIG. 5, 6, 10, 11, 27, 31, 32 or 33 (SEQ ID NOS:
- 1, 2, 3, 4, 5, 6, 7, 8, 45, 47, 48, 50 or 52) or the complementary DNA sequence thereto;
- and
- (b) a DNA sequence encoding an amino acid sequence as set out in FIG. 5, 6, 10, 11, 27, 31, 32 or 33 (SEQ ID NOS:
- 9, 10, 11, 12, 13, 14, 15, 16, 46, 49, 51 or 53) or the complementary DNA sequence thereto.
A purified and isolated nucleic acid molecule consisting of a DNA sequence selected from the group consisting of:
- (a) a DNA sequence as set forth in FIGS. 27, 31, 32 or 33 (SEQ ID Nos:
- 45, 47, 48, 50 or 52) or the complementary DNA sequence thereto;
- and
- (b) a DNA sequence encoding an amino acid sequence as set forth in FIGS. 27, 31, 32 or 33 (SEQ ID Nos:
- 46, 49, 51 or 53) or the complementary sequence thereto.
A purified and isolated nucleic acid molecule consisting of a DNA sequence possessing a restriction map selected from the group consisting of:
- nucleotides 1 to 3225 of M. catarrhalis strain 4223 tbpA gene as set forth in FIG. 3,
- nucleotides 1 to 2106 of M. catarrhalis strain 4223 tbpB gene as set forth in FIG. 4,
- nucleotides 1 to 3660 of M. catarthalis strain Q8 tbpA gene as set forth in FIG. 8,
- nucleotides 1 to 3487 of M. catarrhalis strain Q8 tbpB gene as set forth in FIG. 9,
- nucleotides 1 to 2121 of M. catarrhalis strain M35 tbpB gene as set forth in FIG. 26,
- nucleotides 1 to 2145 of M. catarrhalis strain R1 tbpB gene as set forth in FIG. 28,
- nucleotides 1 to 2129 of M. catarrhalis strain 3 tbpB gene as set forth in FIG. 29, and
- nucleotides 1 to 2142 of M. catarrhalis strain LES1 tbpB gene as set forth in FIG. 30.
A purified and isolated nucleic acid molecule encoding a functional transferrin receptor protein of a strain of Moraxella catarrhalis consisting of a DNA sequence which has at least about 90% sequence identity to any one of the DNA sequences selected from the group consisting of:
- (a) a DNA sequence as set forth in FIG. 5, 6, 10, 11, 27, 31, 32 or 33 (SEQ ID NOS:
- 1, 2, 3, 4, 5, 6, 7, 8, 48, 50, 51, 53 or 55);
- or
- (b) a DNA sequence encoding an amino acid sequence as set forth in FIG. 5, 6, 10, 11, 27, 31, 32 or 33 (SEQ ID NOS:
- 9, 10, 11, 12, 13, 14, 15, 16, 49, 52, 54 or 56).
Dependencies are inferred from the claim text.