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US 6,440,686 B1Granted
Methods for screening and therapeutic applications of kinesin modulators
AssigneeCytokinetics, Inc.
InventorRoman Sakowicz
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Abstract
The present invention relates to protein modulators, especially modulators of kinesin bioactivities. The present invention provides methods for screening protein modulators, e.g., kinesin modulators that bind to a region of a kinesin and/or that modulate kinesin bioactivities. The present invention also provide methods and compositions for treatment of disorders mediated by abnormal cellular proliferation activities.
Claims8
A method for identifying a compound as a KSP modulator, comprising the steps of:
- (a) contacting KSP or a KSP fragment comprising a L5 loop with said compound;
- (b) monitoring fluorescence of a tryptophan residue of said KSP or KSP fragment;
- and
- (c) correlating said fluorescence to modulation of an activity of KSP or said KSP fragment,
- wherein a reduction in fluorescence indicates binding of the compound to KSP or said KSP fragment.
2 more elements
Dependencies are inferred from the claim text.