Methods of identifying modulators of kinesin motor proteins
AssigneeCytokinetics, Inc.
InventorsChristophe Beraud, Jeffrey T. Finer, Roman Sakowicz, Kenneth W. Wood
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Abstract
The present invention provides high throughput screening systems for identifying compounds useful in the treatment of cellular proliferation disorders. The method can be performed in plurality simultaneously with fluorescence or absorbance readouts.
Claims21
A method for screening for modulators of a target protein, wherein the target protein has microtubule stimulated ATPase activity and comprises SEQ ID NO:2, the method comprising the steps of
- contacting the target protein with a candidate agent at a first concentration and determining a level of activity of the target protein;
- and
- contacting the target protein with a candidate agent at a second concentration and determining a level of activity of the target protein;
- wherein the activity is selected from the group consisting of binding activity or ATPase activity, and wherein a difference between the level of activity of the target protein contacted with the first concentration of the candidate agent and the level of activity of the target protein contacted with the second concentration of the candidate agent indicates that the candidate agent modulates the activity of the target protein.
A method for screening for modulators of a target protein, wherein the target protein has microtubule stimulated ATPase activity and comprises SEQ ID NO:4, the method comprising the steps of:
- contacting the target protein with a candidate agent at a first concentration and determining a level of activity of the target protein;
- and
- contacting the target protein with a candidate agent at a second concentration and determining a level of activity of the target protein;
- wherein the activity is selected from the group consisting of binding activity or ATPase activity, and wherein a difference between the level of activity of the target protein contacted with the first concentration of the candidate agent and the level of activity of the target protein contacted with the second concentration of the candidate agent indicates that the candidate agent modulates the activity of the target protein.
A method for screening for modulators of a target protein, wherein the target protein has microtubule stimulated ATPase activity and comprises SEQ ID NO:6, the method comprising the steps of:
- contacting the target protein with a candidate agent at a first concentration and determining a level of activity of the target protein;
- and
- contacting the target protein with a candidate agent at a second concentration and determining a level of activity of the target protein;
- wherein the activity is selected from the group consisting of binding activity or ATPase activity, and wherein a difference between the level of activity of the target protein contacted with the first concentration of the candidate agent and the level of activity of the target protein contacted with the second concentration of the candidate agent indicates that the candidate agent modulates the activity of the target protein.
Dependencies are inferred from the claim text.