Cytosolic phospholipase A2-gamma enzymes and polynucleotides encoding same
AssigneeGenetics Institute, Inc.
InventorsChuanzheng Song, Ronald Kriz, John Knopf
Life of this patent
Estimating the term…
Abstract
The invention provides a novel calcium-independent cytosolic phospholipase A2-Gamma enzyme, polynucleotides encoding such enzyme and methods for screening unknown compounds for anti-inflammatory activity mediated by the arachidonic acid cascade.
Claims6
A method for identifying an inhibitor of phospholipase activity, said method comprising:
- a) contacting a phospholipid with a candidate inhibitor compound and a phospholipase enzyme peptide, wherein the phospholipase enzyme peptide comprises an amino acid sequence selected from:
- i) the amino acid sequence of SEQ ID NO:2;
- and
- ii) a fragment of the amino acid sequence of SEQ ID NO:2 having activity in a mixed micelle assay with 1-palmitoyl-2[14C]-arachidonyl-phosphatidylcholine;
- and
- b) determining whether the phospholipase enzyme peptide cleaves the phospholipid and releases fatty acid,
- thereby identifying an inhibitor of phospholipase activity.
A method for identifying an inhibitor of phospholipase activity, said method comprising:
- a) contacting a phospholipid with a candidate inhibitor compound and a phospholipase enzyme peptide, wherein the phospholipase enzyme peptide comprises an amino acid sequence selected from:
- i) the amino acid sequence of SEQ ID NO:2;
- and
- ii) a fragment of the amino acid sequence of SEQ ID NO:2 having activity in a mixed micelle assay with 1-palmitoyl-2[14C]-arachidonyl-phosphatidylcholine;
- and
- b) determining whether the phospholipase enzyme peptide activity is inhibited by the candidate inhibitor compound,
- thereby identifying an inhibitor of phospholipase activity.
A method for identifying an inhibitor of phospholipase activity, said method comprising:
- a) contacting a phospholipid with a candidate inhibitor compound and a phospholipase enzyme peptide, wherein the phospholipase enzyme peptide comprises the amino acid sequence of SEQ ID NO:2, and
- b) determining whether the phospholipase enzyme peptide activity is inhibited by the candidate inhibitor,
- thereby identifying an inhibitor of phospholipase activity.
A method for identifying an inhibitor of phospholipase activity, said method comprising:
- a) contacting a phospholipid with a candidate inhibitor compound and a phospholipase enzyme peptide, wherein the phospholipase enzyme peptide comprises a fragment of the amino acid sequence of SEQ ID NO:2 having activity in a mixed micelle assay with 1-palmitoyl-2[14C]-arachidonyl-phosphatidylcholine, and
- b) determining whether the phospholipase enzyme peptide activity is inhibited by the candidate inhibitor,
- thereby identifying an inhibitor of phospholipase activity.
A method for identifying an inhibitor of phospholipase activity, said method comprising:
- a) contacting a phospholipid with a candidate inhibitor compound and a phospholipase enzyme peptide, wherein the phospholipase enzyme peptide comprises the amino acid sequence of SEQ ID NO:2;
- and
- b) determining whether the phospholipase enzyme peptide cleaves the phospholipid and releases fatty acid,
- thereby identifying an inhibitor of phospholipase activity.
A method for identifying an inhibitor of phospholipase activity, said method comprising:
- a) contacting a phospholipid with a candidate inhibitor compound and a phospholipase enzyme peptide, wherein the phospholipase enzyme peptide comprises a fragment of the amino acid sequence of SEQ ID NO:2 having activity in a mixed micelle assay with 1-palmitoyl-2[14C]-arachidonyl-phosphatidylcholine;
- and
- b) determining whether the phospholipase enzyme peptide cleaves the phospholipid and releases fatty acid,
- thereby identifying an inhibitor of phospholipase activity.
Dependencies are inferred from the claim text.