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US 6,440,675 B1Granted
Methods for selecting primers
AssigneeWhitehead Institute for Biomedical Research
InventorsKerstin A. Lindblad-Toh, Ellen Winchester, Joel N. Hirschhorn, Pamela Sklar
Life of this patent
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Abstract
Methods for selecting primers having a reduced incidence of undesirable interactions are disclosed. Methods of improving the accuracy of SBE-fluorescence polarization (SBE-FP) methods are also disclosed.
Claims2
A method for selecting primers which have a reduced incidence of undesired interactions comprising identifying primers in which:
- a) at least 4 nucleotides of the primer exhibit reverse complementarity to 4 nucleotides elsewhere on the primer;
- b) there exists a span of sufficient size between the nucleotides that exhibit reverse complementarity to allow the primer to fold back on itself;
- and
- c) there is at least one nucleotide 5′ of the reverse complementary nucleotides which are the most 5′ in said primer,
- wherein identified primers are excluded and the remaining primers have a reduced incidence of undesired interactions.
2 more elements
A method for selecting primers which have a reduced incidence of undesired interactions comprising identifying primers in which:
- a) at least 4 nucleotides of the primer exhibit reverse complementarity to 4 nucleotides elsewhere on the primer;
- b) there exist at least 2 nucleotides between the nucleotides that exhibit reverse complementarity;
- and
- c) there is at least one nucleotide 5′ of the reverse complementary nucleotides which are the most 5′ in said primer,
- wherein identified primers are excluded and the remaining primers have a reduced incidence of undesired interactions.
2 more elements
Dependencies are inferred from the claim text.