High molecular weight major outer membrane protein of moraxella
AssigneeAventis Pasteur Limited
InventorsKen Sasaki, Robin E. Harkness, Sheena M. Loosmore, Michel H. Klein
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Abstract
An isolated and purified outer membrane protein of a Moraxella strain, particularly M. catarrhalis, has a molecular mass of about 200 kDa. The about 200 kDa outer membrane protein as well as nucleic acid molecules encoding the same are useful in diagnostic applications and immunogenic compositions, particularly for in vivo administration to a host to confer protection against disease caused by a bacterial pathogen that produces the about 200 kDa outer membrane protein or produces a protein capable of inducing antibodies in a host specifically reactive with the about 200 kDa outer membrane protein.
Claims13
An immunogenic composition, comprising at least one active component selected from the group consisting of:
- (A) an isolated and purified outer membrane protein of a strain of Moraxella catarrhalis having a molecular mass of about 200 kDa, as determined by SDS-PAGE;
- and
- (B) a peptide consisting of the amino acid sequence NH2-Asn-Val-Lys-Ser-Val-Ile-Asn-Lys-Glu-Gln-Val-Asn-Asp-Ala-Asn-Lys-x-Gln-Gly-Ile (SEQ ID No:
- 2) or NH2-Asn-Val-Lys-Ser-Val-Ile-Asn-Lys-Glu-Gln-Val-Asn-Asp-Ala-Asn-Lys (SEQ ID No:
- 3);
- and a pharmaceutically acceptable carrier therefor, said at least one active component producing an immune response when administered to a host.
A method of producing an isolated and purified outer membrane protein of a strain of Moraxella catarrhalis having a molecular mass of about 200 kDa, as determined by SDS-PAGE, comprising the steps of:
- (a) providing a cell mass of the Moraxella catarrhalis strain;
- (b) disrupting the cell mass to provide a cell lysate;
- (c) fractionating the cell lysate to provide a fraction containing the outer membrane protein substantially free from other cell lysate components, and
- (d) recovering said outer membrane protein.
Dependencies are inferred from the claim text.