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US 6,440,296 B1Granted
Microstructured biosensor, use of the biosensor and process for the immobilization of biocatalysts
AssigneeSiemens Aktiengesselschaft
InventorsManfred Stanzel, Walter Gumbrecht
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Abstract
A biosensor is used for measuring principal metabolites in blood and other substances. The biosensor has a special structure formed of a two-layer enzymatic membrane with a considerably increased functionality compared with enzymatic membranes of the prior art.
Claims7
A microstructured, planar biosensor, in which a biomolecular turnover to be measured can be determined electrochemically, the biosensor comprising:
- a planar substrate;
- a first, inner limiting structure defining a first interior space disposed on said planar substrate;
- an enzymatic layer having an enzyme in a crystalline form disposed in said first interior space;
- a second, outer limiting structure defining a second interior space disposed on said planar substrate;
- and
- a polymeric hydrophilic covering layer disposed in said second interior space completely covering said first, inner limiting structure containing said enzymatic layer.
3 more elements
A method for testing for principal metabolites in blood, which comprises:
- providing a biosensor formed of a planar substrate, a first, inner limiting structure defining a first interior space disposed on the planar substrate, an enzymatic layer having an enzyme in a crystalline form disposed in the first interior space, a second, outer limiting structure defining a second interior space disposed on the planar substrate;
- and a polymeric hydrophilic covering layer disposed in the second interior space completely covering the first, inner limiting structure containing the enzymatic layer;
- and
- using the biosensor for minimally invasive determination of a substrate selected from the group consisting of glucose and lactate in blood.
1 more element
A process for immobilization of enzymes, which comprises:
- filling an enzyme, in a form of a liquid solution, into a first, inner limiting structure on a substrate;
- drying the enzyme initially in the form of the liquid solution into a crystalline form;
- and
- coating the enzyme in the crystalline form with a polymerizable hydrophilic covering layer.
1 more element
Dependencies are inferred from the claim text.