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US 6,437,117 B1Granted
Synthesis, deprotection, analysis and purification for RNA and ribozymes
AssigneeRibozyme Pharmaceuticals, Inc.
InventorsNassim Usman, Francine Wincott, David Sweedler, Leonid Beigelman, Lech W. Dudycz, Susan Grimm, Anthony DiRenzo, Danuta Tracz
Life of this patent
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Abstract
Method for purification and synthesis of RNA molecules and enzymatic RNA molecules in enzymatically active form.
Claims16
A method for purifying an enzymatic RNA molecule of a length of about 28-70 nucleotides, the method comprising:
- a) loading a mixture comprising said RNA onto an anion exchange high-performance liquid chromatography (HPLC) column;
- b) eluting said RNA by passing a buffer through said column to form an eluent;
- and
- c) collecting the eluent from said column and recovering said RNA from said eluent.
1 more element
A method for purifying an enzymatic RNA molecule of a length of about 28-70 nucleotides, the method comprising:
- a) loading a mixture comprising a 5′-protected RNA onto a reverse phase high-performance liquid chromatography (HPLC) column;
- b) eluting said 5′-protected RNA by passing a buffer through said reverse phase column;
- c) removing the 5′-protecting group from said protected RNA to form a deprotected RNA molecule;
- d) loading said deprotected RNA molecule lacking said 5′-protecting group on to an anion exchange high-performance liquid chromatography (HPLC) column;
- e) eluting said RNA by passing a buffer through said anion exchange column to form an eluent;
- and
- f) collecting the eluent from said anion exchange column and recovering said enzymatic RNA molecule from said eluent.
4 more elements
Dependencies are inferred from the claim text.