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AssigneeBoard of Regents, The University of Texas System
InventorsSteven J. Norris, Jing-Ren Zhang, John M. Hardham, Jerrilyn K. Howell, Alan G. Barbour, George M. Weinstock
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The present invention relates to DNA sequences encoding Vmp-like polypeptides of pathogenic Borrelia, the use of the DNA sequences in recombinant vectors to express polypeptides, the encoded amino acid sequences, application of the DNA and amino acid sequences to the production of polypeptides as antigens for immunoprophylaxis, immunotherapy, and immunodiagnosis. Also disclosed are the use of the nucleic acid sequences as probes or primers for the deletion of organisms causing Lyme disease, relapsing fever, or related disorders, and kits designed to facilitate methods of using the described polypeptides, DNA segments and antibodies.
An isolated and purified nucleic acid segment that encodes a polypeptide comprising an amino acid residue sequence of SEQ ID NO:2, or its complement.
An isolated nucleic acid segment comprising at least 15 contiguous bases of SEQ ED NO:1, or the complement thereof.
An isolated nucleic acid segment comprising at least 15 contiguous nucleotides from SEQ ID NO:1 or its complement, wherein said nucleic acid segment is further defined as at least 100 bases in length.
A recombinant host cell comprising a nucleic acid segment comprising at least 20 contiguous bases of SEQ ID NO:1, or the complement thereof.
An expression vector comprising a nucleic acid segment that encodes a polynucleotide comprising at least 15 contiguous bases of SEQ ID NO:
An isolated and purified nucleic acid segment comprising at least nucleotides 75 to 1142 of SEQ ID NO:1, or the complement thereof.
A recombinant host cell comprising a nucleic acid segment comprising at least nucleotides 75 to 1142 of SEQ ID NO:1, or the complement thereof.
An expression vector comprising a nucleic acid segment comprising at least nucleotides 75 to 1142 of SEQ ID NO:1, or the complement thereof.
A method for diagnosing Lyme disease in a subject, comprising assaying for a nucleic acid segment having a nucleotide sequence comprising at least 15 contiguous bases of SEQ ID NO:1, or its complement.
Dependencies are inferred from the claim text.