Optical diffraction biosensor
AssigneeKimberly-Clark Worldwide, Inc.
InventorsDennis S. Everhart, Mark L. Jones, Rosann Marie Kaylor
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Abstract
The present invention provides an inexpensive and sensitive device and method for detecting and quantifying analytes present in a medium. The device comprises a metalized film upon which is printed a specific, predetermined pattern of analyte-specific receptors. Upon attachment of a target analyte to select areas of the plastic film upon which the receptor is printed, diffraction of transmitted and/or reflected light occurs via the physical dimensions and defined, precise placement of the analyte. A diffraction image is produced which can be easily seen with the eye or, optionally, with a sensing device.
Claims37
A method of detecting an analyte comprising:
- contacting the analyte with a biosensor, the biosensor consisting essentially of:
- a polymer film coated with metal;
- and
- a patterned receptor layer printed onto the polymer film wherein
- the receptor layer has a receptive material thereon that
- specifically binds the analyte;
- wherein the receptor layer is printed in a pattern such that when the biosensor binds the analyte, the biosensor diffracts transmitted light or reflected light to form a diffraction pattern.
A method of detecting an analyte comprising
- a. contacting the analyte with a biosensor, the biosensor consisting essentially of:
- a polymer film coated with metal;
- and
- a patterned receptor layer printed onto the polymer film
- wherein the receptor layer has a first receptive material thereon that specifically binds the analyte to form an analyte/first receptive material conjugate;
- b. contacting the biosensor with the analyte/first receptive material conjugate with a second receptor material that specifically binds the analyte/first receptive material conjugate, wherein the second receptor material is conjugated to a precipitate forming material;
- c. contacting the biosensor from step b. with a reagent that will cause a precipitate to form;
- d. wherein the receptor layer is printed in a pattern such that when the biosensor binds the analyte, the biosensor diffracts transmitted light or reflected light to form a diffraction pattern.
Dependencies are inferred from the claim text.