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AssigneeThe Ohio State University Research Foundation
InventorsYasuko Rikihisa, Ning Zhi, Norio Ohashi
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The present invention relates to diagnostic tools for serodiagnosing HGE. The diagnostic tools are structurally related proteins, the “Group 44 proteins”, and to antibodies to such proteins. the Group 44 proteins comprise a single central hypervariable region of approximately 94 amino acid residues, a first conserved region and a second conserved region which flank the central hypervariable region. The hypervariable region of each Group 44 protein has a higher hydrophilicity, a higher antigenic index, and a higher surface probability than either the first conserved region or the second conserved region of the respective protein. The hypervariable region is basic and has an isoelectric point, i.e., a pI, of from about 7.1 to about 9.2 and a molecular mass, i.e., an Mr, of from about 8.5 kDa to about 11 kDa. The Group 44 proteins comprise the P44 protein, the P44-2 protein, the P44-12 protein, the P44-15 protein, the P44-18 protein, and the P44-19 protein and variants of such proteins. The present invention also provides isolated polynucleotides or nucleic acids, referred to collectively hereinafter as the “Group 44 polynucleotides”, which encode the Group 44 proteins and fragments thereof. The present invention provides synthetic oligopeptides of 14-16 amino acids in length. Each of the oligopeptides comprise a sequence which is specific to one Group 44 protein. The present invention also relates to antibodies which are immunospecific for and bind to members of the P44 family of proteinss. The present invention also relates to kits containing reagents for diagnosing HGE.
An isolated outer membrane protein of the human granulocytic ehrlichiosis causative agent, said protein comprising:
An isolated outer membrane protein of the causative agent to human granulocytic ehrlichiosis, wherein said protein is selected from the group consisting of the P44 protein or a variant thereof;
An isolated polypeptide for producing antibodies immunospecific for a Group 44 protein, said polypeptide comprising a sequence which is at least 98% identical to an amino acid sequence selected from the group consisting of the amino acid sequence, SEQ ID NO:2, shown in FIG. 1;
An isolated polypeptide for producing antibodies immunospecific for a Group 44 protein, wherein said isolated polypeptide is 250 amino acids in length or less and comprises a sequence which is identical to the sequence of the hypervariable region of the P44 protein, the P44-2 protein, the P44-12 protein, the P44-15 protein, the P44-18 protein, or the P44-19 protein.
An isolated polypeptide for producing antibodies immunospecific for a Group 44 protein, wherein said polypeptide comprises amino acid 30 through amino acid 248 of the P44 protein or amino acid 38 through amino acid 248 of the P44 protein.
An isolated polypeptide for producing antibodies immunospecific for a Group 44 protein, wherein said polypeptide is 14 to 16 amino acids in length and comprises from 14 to 16 consecutive amino acids within
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