Transplantable recellularized and reendothelialized vascular tissue graft
AssigneeBioscience Consultants
InventorLloyd Wolfinbarger, Jr.
Life of this patent
Estimating the term…
Abstract
The invention provides a device and process for recellularizing essentially acellular or devitalized tissue grafts, including for example essentially acellular or devitalized vascular tissue grafts, derived from human or animal sources, or as constructed using any number of tissue engineering methodologies. The process includes repopulating and reendothelializing an essentially acellular or devitalized tissue graft. The device is useful for producing a repopulated tissue graft from an essentially acellular or devitalized tissue, as well as for producing an essentially acellular or devitalized tissue graft.
Claims74
A process for generating a transplantable recellularized and reendothelialized tissue graft, comprising:
- harvesting tissue from a donor or producing tissue by tissue engineering methods;
- eliminating native cells from said tissue to provide a tissue matrix;
- removing basement membrane from a luminal surface of said tissue matrix;
- treating said tissue matrix with factors to produce a treated tissue matrix;
- recellularizing said treated tissue matrix with a fibroblastic or myofibroblastic cell population to produce a recellularized tissue matrix;
- reendothelializing a luminal surface of said recellularized tissue matrix with an endothelial cell population to produce a repopulated tissue matrix;
- and
- preconditioning said repopulated tissue matrix to promote cell to cell communication, retard apoptotic events, and orient molecular structure of said repopulated tissue matrix to produce said transplantable recellularized and reendothelialized tissue graft.
A process for producing a repopulated, reendothelialized, vascular tissue graft, comprising:
- obtaining a vascular tissue sample;
- eliminating native cellular and extracellular components from said vascular tissue sample, to produce an acellular tissue matrix;
- treating said acellular tissue matrix with cell growth factors to produce a treated matrix;
- removing basement membrane from a luminal surface of said treated matrix to produce a matrix structure;
- repopulating said matrix structure with a fibroblastic cell population to produce a repopulated matrix;
- reendothelializing said luminal surface of said repopulated matrix with an endothelial cell population to produce a reendothelialized matrix;
- and
- preconditioning said reendothelizlized matrix to promote cell to cell communication and retard apoptotic events, to produce said repopulated, reendothelialized, vascular tissue graft.
A process for producing a repopulated, reendothelialized, vascular tissue graft, comprising:
- obtaining a vascular tissue sample;
- eliminating or devitalizing native cellular and extracellullar components from said vascular tissue sample, to produce a tissue matrix;
- treating said tissue matrix with cell growth factors to produce a treated matrix;
- removing basement membrane from a luminal surface of said treated matrix to produce a matrix structure;
- repopulating said matrix structure with a fibroblastic cell population to produce a repopulated matrix;
- reendothelializing said luminal surface of said repopulated matrix with an endothelial cell population to produce a reendothelialized matrix;
- and
- preconditioning said reendothelizlized matrix to promote cell to cell communication and retard apoptotic events, to produce said repopulated, reendothelialized, vascular tissue graft.
A process for producing a repopulated, reendothelialized, vascular tissue graft, comprising:
- obtaining a vascular tissue sample;
- eliminating native cellular and extracellular components from said vascular tissue sample, to produce an acellular tissue matrix;
- treating said acellular tissue matrix with cell growth factors to produce a treated matrix;
- removing basement membrane from a luminal surface of said treated matrix to produce a matrix structure;
- repopulating said matrix structure with a fibroblastic cell population to produce a repopulated matrix;
- reendothelializing said luminal surface of said repopulated matrix with an endothelial cell population to produce a reendothelialized matrix;
- preconditioning said reendothelizlized matrix to promote cell to cell communication and retard apoptotic events, to produce said vascular tissue graft.
A process for generating transplantable recellularized and reendothelialized tissue graft, comprising:
- decellularizing or devitalizing a tissue sample to produce a matrix structure;
- treating said matrix structure with factors to promote viability and function of cells repopulating said matrix structure to produce a treated matrix;
- removing the basement membrane from a luminal surface of said treated matrix;
- recellularizing said membrane-free matrix with a fibroblastic cell population to produce a fibroblastic matrix;
- reendothelializing said luminal surface of said fibroblastic matrix with an endothelial cell population to produce a repopulated tissue;
- and
- preconditioning said repopulated tissue to promote cell to cell communication and retard apoptotic events, to produce said tissue graft.
A process for producing a recellularized and reendothelialized vascular tissue graft comprising:
- obtaining a vascular tissue sample;
- eliminating native cellular components from said vascular tissue sample to produce an acellular tissue matrix;
- removing basement membrane from a luminal surface of said acellular tissue matrix;
- treating said acellular tissue matrix with cell growth factors to produce a treated matrix;
- recellularizing said treated matrix structure with a myofibroblastic cell population to produce a recellularized matrix;
- reendothelializing the luminal surface of said recellularized matrix to produce a repopulated matrix;
- preconditioning said repopulated matrix to promote cell to cell communication, retard apopotosis, and orienate molecular structure of said repopulated matrix to produce said recellularized and reendothelialized vascular tissue graft.
A process for producing a repopulated and reendothelialized vascular tissue graft comprising:
- obtaining a vascular tissue sample;
- eliminating native cellular components from said vascular tissue sample to produce a tissue matrix;
- treating said tissue matrix with cell growth factors to produce a treated matrix;
- removing basement membrane from a luminal surface of said treated matrix to produce a tissue matrix;
- repopulating said tissue matrix with a fibroblastic cell population to produce a repopulated matrix;
- reendothelializing a luminal surface of said repopulated matrix to produce a reendothelialized matrix;
- and
- preconditioning said repopulated and reendothelialized matrix to promote cell to cell communication, retard apoptosis, and orient molecular structure to produce said repopulated and reendothelialized vascular tissue graft.
Dependencies are inferred from the claim text.