Encapsidation cell lines and expression vectors for transcomplementation of defective retroviral vectors
AssigneeOdile Cohen-Haguenauer
InventorOdile Cohen-Haguenauer
Life of this patent
Estimating the term…
Abstract
The present invention relates to novel cell lines, so-called transcomplementation cell lines, which enable the packaging of recombinant retroviral RNAs carrying nucleotide sequences of interest. The present invention aims at transferring and expressing these sequences in eucaryotic target cells. The invention also related to expression vectors for the transcomplementation of defective retroviral vectors.
Claims58
A cultured eukaryotic cell exhibiting the following properties:
- the cell is selected from a dog,
- the cell has a homogeneous morphology that is stable over time,
- the cell is not of tumor origin,
- the cell is selectable in a minimum culture medium lacking CO2, without prior transformation, and
- the cell exhibits accelerated kinetics,
- wherein the cell is suitable for packaging recombinant retroviral RNAs by transcomplementation.
An expression vector comprising:
- a) an env nucleotide sequence coding for one or more polypeptides from a retrovirus of the spumavirus family and permitting the packaging of retroviral RNAs;
- and
- b) transcription regulating signals controlling the expression of the env sequence;
- wherein the expression vector is used for transcomplementation of a retroviral vector, which transfers, integrates, or both, a nucleotide sequence within the genome of a target cell.
An expression vector comprising an env nucleotide sequence, coding for the envelope 4070A of Moloney leukemia virus (Mo-MuLV), said env nucleotide sequence being under the control of an inducible promoter.
An expression vector comprising an env nucleotide sequence, coding for envelope polypeptides from a retrovirus, said env nucleotide sequence being under the control of a RAR-β promoter.
An expression vector comprising:
- a) a gag nucleotide sequence coding for one or more nucleoprotein polypeptides of a Friend retrovirus;
- b) a pol nucleotide sequence coding for one or more pol polypeptides and including a reverse transcriptase protein and an integrase from a Friend retrovirus;
- and
- c) non-viral transcription regulating signals controlling the expression of the gag and pol sequences,
- wherein the vector is used for the transcomplementation of a retroviral vector, that transfers and/or integrates a nucleotide sequence within the genome of a target cell.
An expression vector comprising an env nucleotide sequence, coding for envelope polypeptides from a retrovirus, said env nucleotide sequence being under the control of the LTR sequence of Friend retrovirus.
An expression vector comprising an env nucleotide sequence, coding for envelope polypeptides from a spumavirus, said env nucleotide sequence being under the control of an inducible promoter.
Dependencies are inferred from the claim text.