Viral vectors having chimeric envelope proteins containing the IgG-binding domain of protein A
AssigneeNew York University
InventorsDaniel Meruelo, Kouichi Ohno
Life of this patent
Estimating the term…
Abstract
The invention involves viral vectors that can be used to transduce a target cell, i.e., to introduce genetic material into the cell. The targets of interest are eukaryotic cells and particularly human cells. The transduction can be done in vivo or in vitro. More particularly the invention concerns viral vectors that have chimeric envelope proteins and contain the IgG-binding domain of protein A. These vectors when used in conjunction with antibodies targeting a particular cell are particularly useful for gene therapy.
Claims13
A Sindbis viral vector for transducing a target cell (with high efficiency which comprises a gene encoding a chimeric envelope protein containing an IgG-binding domain of protein A sufficient to bind an Fc domain of an antibody with strong affinity wherein the envelope protein is a Sindbis virus envelope protein;
- wherein the envelope protein is operable to direct the assembly of the protein into a viral particle;
- and wherein said chimeric envelope protein alters natural viral tropism.
A chimeric Sindbis virus for transducing a target cell with high efficiency which comprises a gene of interest under the control of an appropriate viral sequence and a chimeric protein comprising a chimeric Sindbis envelope protein containing an IgG-binding domain of protein A sufficient to bind an Fc domain of an antibody with strong affinity and wherein said chimeric envelope protein alters natural viral tropism.
Dependencies are inferred from the claim text.