Human desaturase gene and uses thereof
AssigneeAbbott Laboratories
InventorsPradip Mukerji, Amanda Eun-Yeong Leonard, Yung-Sheng Huang, Tapas Das
Life of this patent
Estimating the term…
Abstract
The subject invention relates to the identification of a gene involved in the desaturation of polyunsaturated fatty acids at carbon 5 (i.e., “human Δ5-desaturase”) and to uses thereof. In particular, human Δ5-desaturase may be utilized, for example, in the conversion of dihomo-γ-linolenic acid (DGLA) to arachidonic acid (AA) and in the conversion of 20:4n-3 to eicosapentaenoic acid (EPA). AA or polyunsaturated fatty acids produced therefrom may be added to pharmaceutical compositions, nutritional compositions, animal feeds, as well as other products such as cosmetics.
Claims17
An isolated, human nucleotide sequence corresponding to or complementary to at least about 70% of the nucleotide sequence comprising SEQ ID NO:1, wherein said isolated nucleotide sequence encodes a polypeptide comprising SEQ ID NO:12, which desaturates polyunsaturated fatty acids at carbon 5.
A method of producing a human Δ5-desaturase comprising the steps of:
- a) isolating said nucleotide sequence represented by SEQ ID NO:1 (FIG. 12);
- b) constructing a vector comprising:
- i) said isolated nucleotide sequence operably linked to ii) a promoter;
- c) introducing said vector into a host cell under time and conditions sufficient for expression of said human Δ5-desaturase.
A vector comprising:
- a) a nucleotide sequence as represented by SEQ ID NO:1 (FIG. 12) operably linked to
- b) a promoter.
Dependencies are inferred from the claim text.