Sequence and method for genetic engineering of proteins with cell membrane translocating activity
AssigneeVanderbilt University
InventorsYao-Zhong Lin, John P. Donahue, Mauricio Rojas, ZhongJia Tan
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Abstract
The invention describes a membrane-translocating peptide sequence (MTS) which facilitates entry of polypeptides and proteins into cells. Also described is an isolated nucleotide sequence encoding the membrane-translocating peptide and a method of using this sequence to genetically engineer proteins with cell membrane permeability. The MTS, and the method of genetically engineering proteins with cell membrane permeability, are useful for polypeptide and protein delivery for human and veterinary applications such as vaccine delivery and cancer therapy.
Claims12
An isolated fusion polypeptide comprising:
- a) a membrane-translocating peptide of about 8 to about 50 residues comprising at least eight consecutive residues of SEQ ID NO:
- 1 (Ala-Ala-Val-Leu-Leu-Pro-Val-Leu-Leu-Ala-Ala-Pro), and
- b) a target peptide or a target polypeptide.
Dependencies are inferred from the claim text.