Immunostimulatory nucleic acid molecules for activating dendritic cells
AssigneeUniversity of Iowa Research Foundation
InventorsArthur M. Krieg, Gunther Hartmann
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Abstract
The present invention relates generally to methods and products for activating dendritic cells. In particular, the invention relates to oligonucleotides which have a specific sequence including at least one unmethylated CpG dinucleotide which are useful for activating dendritic cells. The methods are useful for in vitro, ex-vivo, and in vivo methods such as cancer immunotherapeutics, treatment of infectious disease and treatment of allergic disease.
Claims21
A method for activating a dendritic cell, comprising:
- contacting a dendritic cell with an isolated nucleic acid containing at least one unmethylated CpG dinucleotide wherein the nucleic acid is from about 8-80 bases in length in an amount effective to activate a dendritic cell, wherein the method is performed ex vivo.
A method for activating a dendritic cell, comprising:
- contacting a dendritic cell with an isolated nucleic acid containing at least one unmethylated CpG dinucleotide wherein the nucleic acid is from about 8-80 bases in length in an amount effective to activate the dendritic cell, wherein the dendritic cell is an isolated dendritic cell.
A method for activating a dendritic cell, comprising:
- contacting a dendritic cell with an isolated nucleic acid containing at least one unmethylated CpG dinucleotide wherein the nucleic acid is from about 8-80 bases in length in an amount effective to activate the dendritic cell, wherein at least one nucleotide of the isolated nucleic acid has a phosphate backbone modification where in the method is peformed ex vivo.
A method for activating a dendritic cell, comprising:
- contacting a dendritic cell with an isolated nucleic acid containing at least one unmethylated CpG dinucleotide in an amount effective to activate the dendritic cell, wherein the isolated nucleic acid is selected from the group consisting of SEQ ID Nos. 84 and 85.
A method for cancer immunotherapy, comprising:
- administering an activated dendritic cell that expresses a specific cancer antigen to a subject having a cancer including the cancer antigen, wherein the activated dendritic cell is prepared by contacting a dendritic cell with an isolated nucleic acid containing at least one unmethylated CpG dinucleotide wherein the nucleic acid is from about 8-80 bases in length in an amount effective to activate the dendritic cell.
A method for treating an infectious disease, comprising:
- administering an activated dendritic cell that expresses a specific microbial antigen to a subject having an infection with a microorganism including the microbial antigen, wherein the activated dendritic cell is prepared by contacting a dendritic cell with an isolated nucleic acid containing at least one unmethylated CpG dinucleotide wherein the nucleic acid is from about 8-80 bases in length in an amount effective to activate the dendritic cell.
A method for treating an allergy, comprising:
- administering an activated dendritic cell that expresses a specific allergy causing antigen to a subject having an allergic reaction to the allergy causing antigen, wherein the activated dendritic cell is prepared by contacting a dendritic cell with an isolated nucleic acid containing at least one unmethylated CpG dinucleotide wherein the nucleic is from about 8-80 bases in length in an amount effective to activate the dendritic cell.
A method for generating a high yield of dendritic cells, comprising:
- administering an isolated nucleic acid containing at least one unmethylated CpG dinucleotide wherein the nucleic acid is from about 8-80 bases in length in an amount effective for activating dendritic cells to a subject;
- allowing the isolated nucleic acid to activate dendritic cells of the subject;
- and
- isolating dendritic cells from the subject.
A method for causing maturation of a dendritic cell, comprising
- contacting a dendritic cell with an isolated nucleic acid containing at least one unmethylated CpG dinucleotide wherein the nucleic acid is from about 8-80 bases in length in an amount effective to cause maturation of the dendritic cell.
A method for activating a dendritic cell, comprising:
- contacting a dendritic cell with an effective amount to activate a dendritic cell of an isolated nucleic acid containing at least one unmethylated CpG dinucleotide and an antigen.
Dependencies are inferred from the claim text.